Gene expression analysis of microtubers of potato solanum tuberosum l. Induced in cytokinin containing medium and osmotic stress

Lisset Herrera-Isidron, Eliana Valencia-Lozano, Pablo Yamild Rosiles-Loeza, Maria Guadalupe Robles-Hernández, Abigail Napsuciale-Heredia, Jose Luis Cabrera-Ponce

Research output: Contribution to journalArticlepeer-review

Abstract

Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using osmotic stress and cytokinin signaling will help us to elucidate this process. We demonstrate in this work a rapid and efficient protocol for microtuber development and gene expression analysis. Medium with high content of sucrose and gelrite supplemented with 2iP as cytokinin under darkness condition produced the higher quantity and quality of microtubers. Gene expression analysis of genes involved in the two-component signaling system (StHK1), cytokinin signaling, (StHK3, StHP4, StRR1) homeodomains (WUSCHEL, POTH1, BEL5), auxin signaling, ARF5, carbon metabolism (TPI, TIM), protein synthesis, NAC5 and a morphogenetic regulator of tuberization (POTH15) was performed by qPCR real time. Differential gene expression was observed during microtuber development. Gene regulation of two component and cytokinin signaling is taking place during this developmental process, yielding more microtubers. Further analysis of each component is required to elucidate it.

Original languageEnglish
Article number876
JournalPlants
Volume10
Issue number5
DOIs
StatePublished - May 2021

Keywords

  • Cytokinin signaling
  • Microtuberization
  • Potato
  • Solanum tuberosum
  • Two component system

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