Gel mobility shift scanning of the acetate-inducible promoters from Neurospora crassa reveals a common co-inducible DNA-binding protein

The promoter regions of four acetate-inducible genes of Neurospora crassa, acu-3, acu-5, acu-8 and acu-9, have been sequenced. Using a scanning gel mobility shift assay particular DNA regions in each promoter have been shown specifically to bind partially purified protein extracted from acetate-induced mycelia. The protein-binding regions so defined have common sequence motifs, elements of which are similar to those required for acetate induction in Aspergillus nidulans.