TY - JOUR
T1 - Gel dressing based on type i collagen modified with oligourethane and silica for skin wound healing
AU - Muñoz-González, Pedro U.
AU - Lona-Ramos, María C.
AU - Gutiérrez-Verdín, Luis D.
AU - Luévano-Colmenero, Guadalupe H.
AU - Tenorio-Rocha, Fernando
AU - García-Contreras, Rene
AU - González-García, Gerardo
AU - Rosillo-De La Torre, Argelia
AU - Delgado, Jorge
AU - Castellano, Laura E.
AU - Mendoza-Novelo, Birzabith
N1 - Publisher Copyright:
© 2022 IOP Publishing Ltd.
PY - 2022/7
Y1 - 2022/7
N2 - Cutaneous wound healing is a complex process that leads the skin reparation with the formation of scar tissue that typically lacks skin appendages. This fact drives us to find new strategies to improve regenerative healing of the skin. This study outlines, the contribution of colloidal silica particles and oligourethane crosslinking on the collagen material properties and the effect on skin wound healing in rats. We characterized the gel properties that are key for in-situ gelation, which is accomplished by the latent reactivity of oligourethane bearing blocked isocyanate groups to crosslink collagen while entrapping silica particles. The swelling/degradation behavior and the elastic modulus of the composite gel were consistent with the modification of collagen type I with oligourethane and silica. On the other hand, these gels were characterized as scaffold for murine macrophages and human stem cells. The application of a composite gel dressing on cutaneous wounds showed a histological appearance of the recovered skin as intact skin; featured by the epidermis, hair follicles, sebaceous glands, subcutaneous adipose layer, and dermis. The results suggest that the collagen-based composite dressings are promising modulators in skin wound healing to achieve a regenerative skin closure with satisfactory functional and aesthetic scars.
AB - Cutaneous wound healing is a complex process that leads the skin reparation with the formation of scar tissue that typically lacks skin appendages. This fact drives us to find new strategies to improve regenerative healing of the skin. This study outlines, the contribution of colloidal silica particles and oligourethane crosslinking on the collagen material properties and the effect on skin wound healing in rats. We characterized the gel properties that are key for in-situ gelation, which is accomplished by the latent reactivity of oligourethane bearing blocked isocyanate groups to crosslink collagen while entrapping silica particles. The swelling/degradation behavior and the elastic modulus of the composite gel were consistent with the modification of collagen type I with oligourethane and silica. On the other hand, these gels were characterized as scaffold for murine macrophages and human stem cells. The application of a composite gel dressing on cutaneous wounds showed a histological appearance of the recovered skin as intact skin; featured by the epidermis, hair follicles, sebaceous glands, subcutaneous adipose layer, and dermis. The results suggest that the collagen-based composite dressings are promising modulators in skin wound healing to achieve a regenerative skin closure with satisfactory functional and aesthetic scars.
KW - inflammation modulation
KW - modified collagen biomaterials
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=85130440409&partnerID=8YFLogxK
U2 - 10.1088/1748-605X/ac6b70
DO - 10.1088/1748-605X/ac6b70
M3 - Artículo
C2 - 35483345
AN - SCOPUS:85130440409
SN - 1748-6041
VL - 17
JO - Biomedical Materials (Bristol)
JF - Biomedical Materials (Bristol)
IS - 4
M1 - 045005
ER -