Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes

José Pedraza-Chaverrí, Perla D. Maldonado, Omar N. Medina-Campos, Ivonne M. Olivares-Corichi, María De Los Ángeles Granados-Silvestre, Rogelio Hernández-Pando, María E. Ibarra-Rubio

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Reactive oxygen species are involved in gentamicin (GM) nephrotoxicity, and garlic is effective in preventing or ameliorating oxidative stress. Therefore, the effect of garlic on GM nephrotoxicity was investigated in this work. Four groups of rats were studied: (i) fed normal diet (CT), (ii) treated with GM (GM), (iii) fed 2% garlic diet (GA), and (iv) treated with GM and 2% garlic diet (GM + GA). Rats were placed in metabolic cages and GM nephrotoxicity was induced by injections of GM (75 mg/kg every 12 h) for 6 d. Lipoperoxidation and enzyme determinations were made in renal cortex on day 7. GM nephrotoxicity was made evident on day 7 by (i) tubular histological damage, (ii) enhanced BUN and urinary excretion of N-acetyl-β-D-glucosaminidase, and (iii) decreased creatinine clearance. These alterations were prevented or ameliorated in GM + GA group. The rise in lipoperoxidation and the decrease in Mn-SOD and glutathione peroxidase (GPx) activities observed in the GM group, were prevented in the GM + GA group. Cu,Zn-SOD activity and Mn-SOD and Cu,Zn-SOD content did not change. CAT activity and content decreased in the GM, GA, and GM + GA groups. CAT mRNA levels decreased in the GM group. The protective effect of garlic is associated with the prevention of the decrease of Mn-SOD and GPx activities and with the rise of lipoperoxidation in renal cortex. Copyright (C) 2000 Elsevier Science Inc.
Original languageAmerican English
Pages (from-to)602-611
Number of pages540
JournalFree Radical Biology and Medicine
DOIs
StatePublished - 14 Oct 2000
Externally publishedYes

Fingerprint

Garlic
antioxidants
Gentamicins
enzymes
Antioxidants
diets
Enzymes
glutathione
cortexes
rats
manganese peroxidase
creatinine
excretion
Nutrition
clearances
Superoxide Dismutase
Glutathione Peroxidase
Diet
injection
Rats

Cite this

Pedraza-Chaverrí, J., Maldonado, P. D., Medina-Campos, O. N., Olivares-Corichi, I. M., Granados-Silvestre, M. D. L. Á., Hernández-Pando, R., & Ibarra-Rubio, M. E. (2000). Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes. Free Radical Biology and Medicine, 602-611. https://doi.org/10.1016/S0891-5849(00)00354-3
Pedraza-Chaverrí, José ; Maldonado, Perla D. ; Medina-Campos, Omar N. ; Olivares-Corichi, Ivonne M. ; Granados-Silvestre, María De Los Ángeles ; Hernández-Pando, Rogelio ; Ibarra-Rubio, María E. / Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes. In: Free Radical Biology and Medicine. 2000 ; pp. 602-611.
@article{edafe26c308a45948e2c5e38ee604969,
title = "Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes",
abstract = "Reactive oxygen species are involved in gentamicin (GM) nephrotoxicity, and garlic is effective in preventing or ameliorating oxidative stress. Therefore, the effect of garlic on GM nephrotoxicity was investigated in this work. Four groups of rats were studied: (i) fed normal diet (CT), (ii) treated with GM (GM), (iii) fed 2{\%} garlic diet (GA), and (iv) treated with GM and 2{\%} garlic diet (GM + GA). Rats were placed in metabolic cages and GM nephrotoxicity was induced by injections of GM (75 mg/kg every 12 h) for 6 d. Lipoperoxidation and enzyme determinations were made in renal cortex on day 7. GM nephrotoxicity was made evident on day 7 by (i) tubular histological damage, (ii) enhanced BUN and urinary excretion of N-acetyl-β-D-glucosaminidase, and (iii) decreased creatinine clearance. These alterations were prevented or ameliorated in GM + GA group. The rise in lipoperoxidation and the decrease in Mn-SOD and glutathione peroxidase (GPx) activities observed in the GM group, were prevented in the GM + GA group. Cu,Zn-SOD activity and Mn-SOD and Cu,Zn-SOD content did not change. CAT activity and content decreased in the GM, GA, and GM + GA groups. CAT mRNA levels decreased in the GM group. The protective effect of garlic is associated with the prevention of the decrease of Mn-SOD and GPx activities and with the rise of lipoperoxidation in renal cortex. Copyright (C) 2000 Elsevier Science Inc.",
author = "Jos{\'e} Pedraza-Chaverr{\'i} and Maldonado, {Perla D.} and Medina-Campos, {Omar N.} and Olivares-Corichi, {Ivonne M.} and Granados-Silvestre, {Mar{\'i}a De Los {\'A}ngeles} and Rogelio Hern{\'a}ndez-Pando and Ibarra-Rubio, {Mar{\'i}a E.}",
year = "2000",
month = "10",
day = "14",
doi = "10.1016/S0891-5849(00)00354-3",
language = "American English",
pages = "602--611",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",

}

Pedraza-Chaverrí, J, Maldonado, PD, Medina-Campos, ON, Olivares-Corichi, IM, Granados-Silvestre, MDLÁ, Hernández-Pando, R & Ibarra-Rubio, ME 2000, 'Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes', Free Radical Biology and Medicine, pp. 602-611. https://doi.org/10.1016/S0891-5849(00)00354-3

Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes. / Pedraza-Chaverrí, José; Maldonado, Perla D.; Medina-Campos, Omar N.; Olivares-Corichi, Ivonne M.; Granados-Silvestre, María De Los Ángeles; Hernández-Pando, Rogelio; Ibarra-Rubio, María E.

In: Free Radical Biology and Medicine, 14.10.2000, p. 602-611.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes

AU - Pedraza-Chaverrí, José

AU - Maldonado, Perla D.

AU - Medina-Campos, Omar N.

AU - Olivares-Corichi, Ivonne M.

AU - Granados-Silvestre, María De Los Ángeles

AU - Hernández-Pando, Rogelio

AU - Ibarra-Rubio, María E.

PY - 2000/10/14

Y1 - 2000/10/14

N2 - Reactive oxygen species are involved in gentamicin (GM) nephrotoxicity, and garlic is effective in preventing or ameliorating oxidative stress. Therefore, the effect of garlic on GM nephrotoxicity was investigated in this work. Four groups of rats were studied: (i) fed normal diet (CT), (ii) treated with GM (GM), (iii) fed 2% garlic diet (GA), and (iv) treated with GM and 2% garlic diet (GM + GA). Rats were placed in metabolic cages and GM nephrotoxicity was induced by injections of GM (75 mg/kg every 12 h) for 6 d. Lipoperoxidation and enzyme determinations were made in renal cortex on day 7. GM nephrotoxicity was made evident on day 7 by (i) tubular histological damage, (ii) enhanced BUN and urinary excretion of N-acetyl-β-D-glucosaminidase, and (iii) decreased creatinine clearance. These alterations were prevented or ameliorated in GM + GA group. The rise in lipoperoxidation and the decrease in Mn-SOD and glutathione peroxidase (GPx) activities observed in the GM group, were prevented in the GM + GA group. Cu,Zn-SOD activity and Mn-SOD and Cu,Zn-SOD content did not change. CAT activity and content decreased in the GM, GA, and GM + GA groups. CAT mRNA levels decreased in the GM group. The protective effect of garlic is associated with the prevention of the decrease of Mn-SOD and GPx activities and with the rise of lipoperoxidation in renal cortex. Copyright (C) 2000 Elsevier Science Inc.

AB - Reactive oxygen species are involved in gentamicin (GM) nephrotoxicity, and garlic is effective in preventing or ameliorating oxidative stress. Therefore, the effect of garlic on GM nephrotoxicity was investigated in this work. Four groups of rats were studied: (i) fed normal diet (CT), (ii) treated with GM (GM), (iii) fed 2% garlic diet (GA), and (iv) treated with GM and 2% garlic diet (GM + GA). Rats were placed in metabolic cages and GM nephrotoxicity was induced by injections of GM (75 mg/kg every 12 h) for 6 d. Lipoperoxidation and enzyme determinations were made in renal cortex on day 7. GM nephrotoxicity was made evident on day 7 by (i) tubular histological damage, (ii) enhanced BUN and urinary excretion of N-acetyl-β-D-glucosaminidase, and (iii) decreased creatinine clearance. These alterations were prevented or ameliorated in GM + GA group. The rise in lipoperoxidation and the decrease in Mn-SOD and glutathione peroxidase (GPx) activities observed in the GM group, were prevented in the GM + GA group. Cu,Zn-SOD activity and Mn-SOD and Cu,Zn-SOD content did not change. CAT activity and content decreased in the GM, GA, and GM + GA groups. CAT mRNA levels decreased in the GM group. The protective effect of garlic is associated with the prevention of the decrease of Mn-SOD and GPx activities and with the rise of lipoperoxidation in renal cortex. Copyright (C) 2000 Elsevier Science Inc.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0033804999&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0033804999&origin=inward

U2 - 10.1016/S0891-5849(00)00354-3

DO - 10.1016/S0891-5849(00)00354-3

M3 - Article

SP - 602

EP - 611

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

ER -

Pedraza-Chaverrí J, Maldonado PD, Medina-Campos ON, Olivares-Corichi IM, Granados-Silvestre MDLÁ, Hernández-Pando R et al. Garlic ameliorates gentamicin nephrotoxicity: Relation to antioxidant enzymes. Free Radical Biology and Medicine. 2000 Oct 14;602-611. https://doi.org/10.1016/S0891-5849(00)00354-3