TY - JOUR
T1 - Frequency of S and Z alleles for alpha-1-antitrypsin and tumor necrosis factor alpha - 308 promoter polymorphism in Northeastern Mexico
AU - Sánchez-Domínguez, Celia Nohemí
AU - Buenfil-Lozano, José Antonio
AU - Molina-Guajardo, Carlos Alejandro
AU - Borjas-Almaguer, Omar David
AU - Castillo-Lartigue, Abraham
AU - Bustamante-Sáenz, Adriana
AU - Martínez-Rodríguez, Herminia Guadalupe
AU - Alarcón, Miguel Ángel Villarreal
AU - Reyes-López, Miguel Ángel
AU - Ortiz-López, Rocío
PY - 2008
Y1 - 2008
N2 - Diseases characterized by airway inflammation, excessive secretion, and obstruction affect a substantial proportion of the population. Studies for understanding the mechanisms underlying these processes are focused on the initiation and maintenance of inflammation. Polymorphisms on DNA sequence of response mediators such as alpha-1-antitrypsin (AAT) and tumor necrosis factor (TNF) alpha have the capacity to influence presentation of diseases, affecting protein amount and/or functionality, and can be analyzed as disease modulators. The purpose of this study was to analyze AAT S and Z alleles and -308G/A TNF-alpha polymorphism on the northeast Mexico mestizo population to compare the influence of these genes in several diseases. DNA samples from 103 volunteers (healthy group) were tested for modifier gene variants by polymerase chain reaction-RFLP as follows: AAT gene for S and Z alleles and TNF-alpha promoter -308G/A (TNF1/TNF2) alleles. Allele frequency for S and TNF2 alleles were 1.5 and 2.4%, respectively, whereas the Z allele was not detected. This study shows low frequencies of the AAT S and TNF2 alleles, and the Z allele was not found. Correlation studies in the future will allow to determine if these alleles have some influence in the clinical presentation of diverse diseases in this group of people.
AB - Diseases characterized by airway inflammation, excessive secretion, and obstruction affect a substantial proportion of the population. Studies for understanding the mechanisms underlying these processes are focused on the initiation and maintenance of inflammation. Polymorphisms on DNA sequence of response mediators such as alpha-1-antitrypsin (AAT) and tumor necrosis factor (TNF) alpha have the capacity to influence presentation of diseases, affecting protein amount and/or functionality, and can be analyzed as disease modulators. The purpose of this study was to analyze AAT S and Z alleles and -308G/A TNF-alpha polymorphism on the northeast Mexico mestizo population to compare the influence of these genes in several diseases. DNA samples from 103 volunteers (healthy group) were tested for modifier gene variants by polymerase chain reaction-RFLP as follows: AAT gene for S and Z alleles and TNF-alpha promoter -308G/A (TNF1/TNF2) alleles. Allele frequency for S and TNF2 alleles were 1.5 and 2.4%, respectively, whereas the Z allele was not detected. This study shows low frequencies of the AAT S and TNF2 alleles, and the Z allele was not found. Correlation studies in the future will allow to determine if these alleles have some influence in the clinical presentation of diverse diseases in this group of people.
KW - AATS
KW - AATZ
KW - Alpha-1-antitrypsin
KW - Cytokines
KW - Mexican population
KW - PCR-RFLP
KW - Polymorphisms
KW - Population genetics
KW - TNF-1
KW - TNF-2
KW - Tumor necrosis factor alpha polymorphism
UR - http://www.scopus.com/inward/record.url?scp=50349086109&partnerID=8YFLogxK
U2 - 10.2500/aap.2008.29.3125
DO - 10.2500/aap.2008.29.3125
M3 - Artículo
SN - 1088-5412
VL - 29
SP - 406
EP - 410
JO - Allergy and Asthma Proceedings
JF - Allergy and Asthma Proceedings
IS - 4
ER -