Fractionation and electrophoretic patterns of seed protein of Opuntia genus. A preliminary survey as a tool for accession differentiation and taxonomy

Samir Samah, Elsa Ventura-Zapata, Ernestina Valadez-Moctezuma

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

At present, little is known about Opuntia seed proteins and their contribution to the characterization and taxonomy of genotypes belong to this genus. The variation among 102 accessions of Mexican Opuntia was studied using electrophoretic patterns by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of their seed total proteins (STPs) and seed storage proteins (SSPs). Albumins and globulins were the most abundant protein fractions, with contents varied from 2.6 to 11.9mg/mL and 2.6-9.5mg/mL, respectively. These were followed by glutelins (2.3-8.5mg/mL) and prolamins as the lowest (1.1-7.9mg/mL) of the four protein factions. On the other hand, STPs content varied between 1.13 and 7.12mg/mL. Regardless of variations in protein content estimated in seeds, the electrophoretic patterns of STPs and SSPs, as outcome of their SDS-PAGE, were not so variable. Furthermore, the individual analysis of each STPs or the SSPs analyses, separately, were not enough to discriminate all accessions, since it was necessary to combine the data resulting from all protein profiles together to differentiate all Opuntia genotypes. The UPGMA analysis indicated that there is no separation between accessions of species of the prickly pear (sweet fruits) and ". xoconostle" (acidic fruits), even though the latter were grouped together. Based on biochemical markers analyzed herein, the need for revision of taxonomic assignment of genotypes belonging to the genus Opuntia is suggested.

Original languageEnglish
Pages (from-to)187-194
Number of pages8
JournalBiochemical Systematics and Ecology
Volume58
DOIs
StatePublished - 1 Feb 2015

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Opuntia
Taxonomies
Fractionation
Seed
Seeds
fractionation
taxonomy
seed
Seed Storage Proteins
protein
seed storage proteins
seeds
Proteins
proteins
Fruits
Electrophoresis
Sodium Dodecyl Sulfate
polyacrylamide gel electrophoresis
Genotype
genotype

Keywords

  • Biochemical markers
  • Characterization
  • Nopal
  • SDS-PAGE

Cite this

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title = "Fractionation and electrophoretic patterns of seed protein of Opuntia genus. A preliminary survey as a tool for accession differentiation and taxonomy",
abstract = "At present, little is known about Opuntia seed proteins and their contribution to the characterization and taxonomy of genotypes belong to this genus. The variation among 102 accessions of Mexican Opuntia was studied using electrophoretic patterns by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of their seed total proteins (STPs) and seed storage proteins (SSPs). Albumins and globulins were the most abundant protein fractions, with contents varied from 2.6 to 11.9mg/mL and 2.6-9.5mg/mL, respectively. These were followed by glutelins (2.3-8.5mg/mL) and prolamins as the lowest (1.1-7.9mg/mL) of the four protein factions. On the other hand, STPs content varied between 1.13 and 7.12mg/mL. Regardless of variations in protein content estimated in seeds, the electrophoretic patterns of STPs and SSPs, as outcome of their SDS-PAGE, were not so variable. Furthermore, the individual analysis of each STPs or the SSPs analyses, separately, were not enough to discriminate all accessions, since it was necessary to combine the data resulting from all protein profiles together to differentiate all Opuntia genotypes. The UPGMA analysis indicated that there is no separation between accessions of species of the prickly pear (sweet fruits) and {"}. xoconostle{"} (acidic fruits), even though the latter were grouped together. Based on biochemical markers analyzed herein, the need for revision of taxonomic assignment of genotypes belonging to the genus Opuntia is suggested.",
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T1 - Fractionation and electrophoretic patterns of seed protein of Opuntia genus. A preliminary survey as a tool for accession differentiation and taxonomy

AU - Samah, Samir

AU - Ventura-Zapata, Elsa

AU - Valadez-Moctezuma, Ernestina

PY - 2015/2/1

Y1 - 2015/2/1

N2 - At present, little is known about Opuntia seed proteins and their contribution to the characterization and taxonomy of genotypes belong to this genus. The variation among 102 accessions of Mexican Opuntia was studied using electrophoretic patterns by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of their seed total proteins (STPs) and seed storage proteins (SSPs). Albumins and globulins were the most abundant protein fractions, with contents varied from 2.6 to 11.9mg/mL and 2.6-9.5mg/mL, respectively. These were followed by glutelins (2.3-8.5mg/mL) and prolamins as the lowest (1.1-7.9mg/mL) of the four protein factions. On the other hand, STPs content varied between 1.13 and 7.12mg/mL. Regardless of variations in protein content estimated in seeds, the electrophoretic patterns of STPs and SSPs, as outcome of their SDS-PAGE, were not so variable. Furthermore, the individual analysis of each STPs or the SSPs analyses, separately, were not enough to discriminate all accessions, since it was necessary to combine the data resulting from all protein profiles together to differentiate all Opuntia genotypes. The UPGMA analysis indicated that there is no separation between accessions of species of the prickly pear (sweet fruits) and ". xoconostle" (acidic fruits), even though the latter were grouped together. Based on biochemical markers analyzed herein, the need for revision of taxonomic assignment of genotypes belonging to the genus Opuntia is suggested.

AB - At present, little is known about Opuntia seed proteins and their contribution to the characterization and taxonomy of genotypes belong to this genus. The variation among 102 accessions of Mexican Opuntia was studied using electrophoretic patterns by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of their seed total proteins (STPs) and seed storage proteins (SSPs). Albumins and globulins were the most abundant protein fractions, with contents varied from 2.6 to 11.9mg/mL and 2.6-9.5mg/mL, respectively. These were followed by glutelins (2.3-8.5mg/mL) and prolamins as the lowest (1.1-7.9mg/mL) of the four protein factions. On the other hand, STPs content varied between 1.13 and 7.12mg/mL. Regardless of variations in protein content estimated in seeds, the electrophoretic patterns of STPs and SSPs, as outcome of their SDS-PAGE, were not so variable. Furthermore, the individual analysis of each STPs or the SSPs analyses, separately, were not enough to discriminate all accessions, since it was necessary to combine the data resulting from all protein profiles together to differentiate all Opuntia genotypes. The UPGMA analysis indicated that there is no separation between accessions of species of the prickly pear (sweet fruits) and ". xoconostle" (acidic fruits), even though the latter were grouped together. Based on biochemical markers analyzed herein, the need for revision of taxonomic assignment of genotypes belonging to the genus Opuntia is suggested.

KW - Biochemical markers

KW - Characterization

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