TY - JOUR
T1 - Fluoxetine-induced neurotoxicity at environmentally relevant concentrations in adult zebrafish Danio rerio
AU - Orozco-Hernández, José Manuel
AU - Gómez-Oliván, Leobardo Manuel
AU - Elizalde-Velázquez, Gustavo Axel
AU - Rosales-Pérez, Karina Elisa
AU - Cardoso-Vera, Jesús Daniel
AU - Heredia-García, Gerardo
AU - Islas-Flores, Hariz
AU - García-Medina, Sandra
AU - Galar-Martínez, Marcela
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/5
Y1 - 2022/5
N2 - Fluoxetine (FLX) exerts its therapeutic effect by inhibiting the presynaptic reuptake of the neurotransmitter serotonin. Nonetheless, at high concentrations of this drug, adverse effects occur in the brain of exposed organisms. Bearing this into account, the objective of this study was to evaluate the neurotoxic effects of the fluoxetine through the evaluation of behavior (Novel tank test), determination of oxidative stress, and determination of acetylcholinesterase (AChE) activity in adult zebrafish Danio rerio. For this purpose, Danio rerio adults were exposed to three environmentally relevant concentrations (5, 10, 16 ng L−1) of FLX for 96 h. Our results demonstrate fish presented a significant disruption in their behavior, as they remained long-lasting time frozen at the top of the tank. Since we observed a significant reduction of AChE activity in the brain of fish, we believe the above described anxiety-like state is the result of this enzyme impairment. Moreover, as FLX-exposed fish showed a significant increase in the levels of oxidative damage biomarkers, we suggest this AChE disruption is associated with the oxidative stress response fish exhibited. Based on our findings, we believe the environmentally relevant concentration of FLX alters the redox status of the brain, impairing this way the behavior of fish and making them more vulnerable to predation.
AB - Fluoxetine (FLX) exerts its therapeutic effect by inhibiting the presynaptic reuptake of the neurotransmitter serotonin. Nonetheless, at high concentrations of this drug, adverse effects occur in the brain of exposed organisms. Bearing this into account, the objective of this study was to evaluate the neurotoxic effects of the fluoxetine through the evaluation of behavior (Novel tank test), determination of oxidative stress, and determination of acetylcholinesterase (AChE) activity in adult zebrafish Danio rerio. For this purpose, Danio rerio adults were exposed to three environmentally relevant concentrations (5, 10, 16 ng L−1) of FLX for 96 h. Our results demonstrate fish presented a significant disruption in their behavior, as they remained long-lasting time frozen at the top of the tank. Since we observed a significant reduction of AChE activity in the brain of fish, we believe the above described anxiety-like state is the result of this enzyme impairment. Moreover, as FLX-exposed fish showed a significant increase in the levels of oxidative damage biomarkers, we suggest this AChE disruption is associated with the oxidative stress response fish exhibited. Based on our findings, we believe the environmentally relevant concentration of FLX alters the redox status of the brain, impairing this way the behavior of fish and making them more vulnerable to predation.
KW - Antidepressant
KW - Behavioral studies
KW - Neurotransmitters
KW - Oxidative status
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85127052411&partnerID=8YFLogxK
U2 - 10.1016/j.neuro.2022.03.007
DO - 10.1016/j.neuro.2022.03.007
M3 - Artículo
C2 - 35304135
AN - SCOPUS:85127052411
SN - 0161-813X
VL - 90
SP - 121
EP - 129
JO - NeuroToxicology
JF - NeuroToxicology
ER -