First report of carrot cyst nematode Heterodera carotae in Mexico: Morphological, molecular characterization and host range study

Ilia Mariana Escobar-Avila, Edgar Óliver López-Villegas, Sergei A. Subbotin, Alejandro Tovar-Soto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these species from each other. Our study showed that male stylet length is longer for H. carotae compared with that for H. cruciferae. This is an example of importance of combination of several methods for the correct identification of cyst nematodes. To our knowledge, this is the first report of H. carotae in Mexico.

Original languageEnglish
Pages (from-to)229-242
Number of pages14
JournalJournal of Nematology
Volume50
Issue number2
DOIs
StatePublished - 1 Jan 2018

Fingerprint

Heterodera carotae
cyst nematodes
host range
carrots
Mexico
Brassicaceae
valleys
Nematoda
stylets
Daucus carota
parasitism
population density
scanning electron microscopy
ribosomal RNA
genes
testing

Keywords

  • Carrot
  • COI gene
  • Daucus carota
  • ITS rRNA gene
  • Morphometrics
  • Phylogeny

Cite this

@article{973fbda3d4d041acb7c776ab4597624e,
title = "First report of carrot cyst nematode Heterodera carotae in Mexico: Morphological, molecular characterization and host range study",
abstract = "During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61{\%} of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these species from each other. Our study showed that male stylet length is longer for H. carotae compared with that for H. cruciferae. This is an example of importance of combination of several methods for the correct identification of cyst nematodes. To our knowledge, this is the first report of H. carotae in Mexico.",
keywords = "Carrot, COI gene, Daucus carota, ITS rRNA gene, Morphometrics, Phylogeny",
author = "Escobar-Avila, {Ilia Mariana} and L{\'o}pez-Villegas, {Edgar {\'O}liver} and Subbotin, {Sergei A.} and Alejandro Tovar-Soto",
year = "2018",
month = "1",
day = "1",
doi = "10.21307/jofnem-2018-021",
language = "Ingl{\'e}s",
volume = "50",
pages = "229--242",
journal = "Journal of Nematology",
issn = "0022-300X",
publisher = "Society of Nematologists",
number = "2",

}

First report of carrot cyst nematode Heterodera carotae in Mexico : Morphological, molecular characterization and host range study. / Escobar-Avila, Ilia Mariana; López-Villegas, Edgar Óliver; Subbotin, Sergei A.; Tovar-Soto, Alejandro.

In: Journal of Nematology, Vol. 50, No. 2, 01.01.2018, p. 229-242.

Research output: Contribution to journalArticle

TY - JOUR

T1 - First report of carrot cyst nematode Heterodera carotae in Mexico

T2 - Morphological, molecular characterization and host range study

AU - Escobar-Avila, Ilia Mariana

AU - López-Villegas, Edgar Óliver

AU - Subbotin, Sergei A.

AU - Tovar-Soto, Alejandro

PY - 2018/1/1

Y1 - 2018/1/1

N2 - During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these species from each other. Our study showed that male stylet length is longer for H. carotae compared with that for H. cruciferae. This is an example of importance of combination of several methods for the correct identification of cyst nematodes. To our knowledge, this is the first report of H. carotae in Mexico.

AB - During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these species from each other. Our study showed that male stylet length is longer for H. carotae compared with that for H. cruciferae. This is an example of importance of combination of several methods for the correct identification of cyst nematodes. To our knowledge, this is the first report of H. carotae in Mexico.

KW - Carrot

KW - COI gene

KW - Daucus carota

KW - ITS rRNA gene

KW - Morphometrics

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=85059141467&partnerID=8YFLogxK

U2 - 10.21307/jofnem-2018-021

DO - 10.21307/jofnem-2018-021

M3 - Artículo

C2 - 30451440

AN - SCOPUS:85059141467

VL - 50

SP - 229

EP - 242

JO - Journal of Nematology

JF - Journal of Nematology

SN - 0022-300X

IS - 2

ER -