TY - GEN
T1 - Feather waste as petroleum sorbent
T2 - 23rd Annual International Conference on Soils Sediments and Water 2007
AU - Rojas-Avelizapa, L. I.
AU - Cervantes-González, E.
AU - Cruz-Camarillo, R.
AU - Rojas-Avelizapa, N. G.
AU - García-Mena, J.
PY - 2007
Y1 - 2007
N2 - Using scanning electron microscopy (SEM), the present study evaluated the biodegradation of chicken feathers during a petroleum hydrocarbon removal process by a defined-mixed culture that pose the simultaneous abilities to remove petroleum hydrocarbons and produce keratinases in liquid culture. Biodegradation treatments were performed in Erlenmeyer flasks containing mineral media, 6% w/v of chicken feathers and 64,800 mg I-1 of petroleum hydrocarbons. Flasks were inoculated with the keratinolytic-mixed culture, which was previously obtained from a petroleum-polluted site, and then incubated at 28°C, 180 rpm during 21 days. Every 7th day, a sample was collected and fractioned; one fraction was processed to be analyzed by SEM while the residual petroleum-hydrocarbons were extracted from the other fraction and quantified by gas chromatography. Controls without inocula were processed under same conditions. The photomicrographs illustrated the different stages of the feathers' biodegradation; they are first found intact without degradation while the microorganisms from the mixed culture appear only in the supernatants. After the 7th day a remarkable colonization of the feathers begins to be observed, along with a considerable degradation observed after the 14th day of incubation.
AB - Using scanning electron microscopy (SEM), the present study evaluated the biodegradation of chicken feathers during a petroleum hydrocarbon removal process by a defined-mixed culture that pose the simultaneous abilities to remove petroleum hydrocarbons and produce keratinases in liquid culture. Biodegradation treatments were performed in Erlenmeyer flasks containing mineral media, 6% w/v of chicken feathers and 64,800 mg I-1 of petroleum hydrocarbons. Flasks were inoculated with the keratinolytic-mixed culture, which was previously obtained from a petroleum-polluted site, and then incubated at 28°C, 180 rpm during 21 days. Every 7th day, a sample was collected and fractioned; one fraction was processed to be analyzed by SEM while the residual petroleum-hydrocarbons were extracted from the other fraction and quantified by gas chromatography. Controls without inocula were processed under same conditions. The photomicrographs illustrated the different stages of the feathers' biodegradation; they are first found intact without degradation while the microorganisms from the mixed culture appear only in the supernatants. After the 7th day a remarkable colonization of the feathers begins to be observed, along with a considerable degradation observed after the 14th day of incubation.
KW - Chicken feathers
KW - Electron microscopy
KW - Keratinases
KW - Sorbent
UR - http://www.scopus.com/inward/record.url?scp=67449131983&partnerID=8YFLogxK
M3 - Contribución a la conferencia
AN - SCOPUS:67449131983
SN - 9781605606255
T3 - Association for Environmental Health and Sciences - 23rd Annual International Conference on Soils, Sediments and Water 2007
SP - 50
EP - 58
BT - Association for Environmental Health and Sciences - 23rd Annual International Conference on Soils, Sediments and Water 2007
Y2 - 15 October 2007 through 18 October 2007
ER -