TY - JOUR
T1 - Feasibility study for the detection of Trichinella spiralis in a murine model using mid-Fourier transform infrared spectroscopy (MID-FTIR) with attenuated total reflectance (ATR) and soft independent modelling of class analogies (SIMCA)
AU - Anda, Fabián Gómez De
AU - Gallardo-Velazquez, Tzayhri
AU - Osorio-Revilla, Guillermo
AU - Dorantes-Alvarez, Lidia
AU - Calderon-Dominguez, Georgina
AU - Nogueda-Torres, Benjamín
AU - De-la-Rosa-Arana, Jorge Luis
N1 - Funding Information:
This work received financial support from the Secretaria de Investigación y Estudios de Posgrado del IPN, México , through the projects SIP-20100575 and SIP-20110813. Mr. Fabián Gómez-De Anda was a grateful recipient of a grant from CONACYT and SIP-IPN. The authors thank R. Jimenez and V. Molina, members of the Tissular Helminths Laboratory, for the technical assistance and to I. Valle and M. Piñon for the care of the animals at the InDRE facilities.
PY - 2012/12/21
Y1 - 2012/12/21
N2 - Fourier transform infrared (FTIR) spectroscopy with attenuated total reflectance (ATR) and soft independent modelling by class analogies (SIMCA) was used to assess the feasibility of detecting Trichinella spiralis in a murine model. The selected FTIR wavenumber range was 1700-900cm-1 and the first derivative of the spectra was subjected to SIMCA analysis. The SIMCA model developed for rat meat spiked with T. spiralis larvae was successfully apply to classify non-infected from infected rat meat with a limit of detection of 3 larvae/10g rat meat and no false positives with 99% confidence limit. To avoid false positives arising from the presence of other parasites, another chemometric model was developed to demonstrate the capacity of the model to discriminate between Ascaris suum, Taenia solium and T. spiralis. Results confirmed that this method could correctly distinguish these parasites. Additional studies are needed to prove the effectiveness of this technique for other types of muscle meats, including those relevant to human consumption.
AB - Fourier transform infrared (FTIR) spectroscopy with attenuated total reflectance (ATR) and soft independent modelling by class analogies (SIMCA) was used to assess the feasibility of detecting Trichinella spiralis in a murine model. The selected FTIR wavenumber range was 1700-900cm-1 and the first derivative of the spectra was subjected to SIMCA analysis. The SIMCA model developed for rat meat spiked with T. spiralis larvae was successfully apply to classify non-infected from infected rat meat with a limit of detection of 3 larvae/10g rat meat and no false positives with 99% confidence limit. To avoid false positives arising from the presence of other parasites, another chemometric model was developed to demonstrate the capacity of the model to discriminate between Ascaris suum, Taenia solium and T. spiralis. Results confirmed that this method could correctly distinguish these parasites. Additional studies are needed to prove the effectiveness of this technique for other types of muscle meats, including those relevant to human consumption.
KW - Diagnosis
KW - FTIR-ATR
KW - SIMCA
KW - Trichinella spiralis
UR - http://www.scopus.com/inward/record.url?scp=84868206044&partnerID=8YFLogxK
U2 - 10.1016/j.vetpar.2012.07.004
DO - 10.1016/j.vetpar.2012.07.004
M3 - Artículo
SN - 0304-4017
VL - 190
SP - 496
EP - 503
JO - Veterinary Parasitology
JF - Veterinary Parasitology
IS - 3-4
ER -