Expression of Antimicrobial Peptides in Human Monocytic Cells and Neutrophils in Response to Dengue Virus Type 2

Jorge I. Castañeda-Sánchez, Diana Alhelí Domínguez-Martínez, Nataly Olivar-Espinosa, Blanca Estela García-Pérez, María Alba Loroño-Pino, Julieta Luna-Herrera, Ma Isabel Salazar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

© 2016 S. Karger AG, Basel. Background/Aims: The innate immune response is remarkably important for controlling infections. Information about the participation of antimicrobial peptides (AMPs) in response to dengue virus (DENV) is scarce. The aim of this study was to examine the AMP response to DENV-2 in human THP-1 cells and neutrophils. Methods: Protein and mRNA levels of two AMPs - hBD-1 and cathelicidin LL-37 - were assessed in DENV-infected macrophage-like THP-1 cells using qRT-PCR and indirect immunofluorescence. Also, mRNA levels of α-defensins (hDEFAs) and LL-37 were examined by qRT-PCR in human neutrophils taken from peripheral blood and treated with DENV-2. Results: mRNA expression of hBD-1 rose in THP-1 cells at 24-72 h, while protein expression increased later, from 48 to 72 h after infection. Cathelic idin LL-37 mRNA expression of DENV-infected THP-1 cells was observed at 6-48 h after infection, while protein levels increased importantly up to 72 h after infection. Regarding neutrophils, the mRNA expression of hDEFAs and LL-37 increased slightly at 2 and 5 h after the contact with DENV-2. Conclusion: THP-1 cells and human neutrophils strongly respond to DENV by producing AMPs: hBD-1 and LL-37 for the THP-1 cells and hDEFAs and LL-37 for neutrophils. However, the direct effect of these molecules on DENV particles remains unclear.
Original languageAmerican English
Pages (from-to)8-19
Number of pages12
JournalIntervirology
DOIs
StatePublished - 1 Sep 2016

Fingerprint

Dengue Virus
Neutrophils
Peptides
Messenger RNA
Infection
Defensins
Polymerase Chain Reaction
Proteins
Indirect Fluorescent Antibody Technique
Innate Immunity
Virion
Macrophages

Cite this

Castañeda-Sánchez, J. I., Domínguez-Martínez, D. A., Olivar-Espinosa, N., García-Pérez, B. E., Loroño-Pino, M. A., Luna-Herrera, J., & Salazar, M. I. (2016). Expression of Antimicrobial Peptides in Human Monocytic Cells and Neutrophils in Response to Dengue Virus Type 2. Intervirology, 8-19. https://doi.org/10.1159/000446282
Castañeda-Sánchez, Jorge I. ; Domínguez-Martínez, Diana Alhelí ; Olivar-Espinosa, Nataly ; García-Pérez, Blanca Estela ; Loroño-Pino, María Alba ; Luna-Herrera, Julieta ; Salazar, Ma Isabel. / Expression of Antimicrobial Peptides in Human Monocytic Cells and Neutrophils in Response to Dengue Virus Type 2. In: Intervirology. 2016 ; pp. 8-19.
@article{807babcfe4574d2d8c938f1dbe59c622,
title = "Expression of Antimicrobial Peptides in Human Monocytic Cells and Neutrophils in Response to Dengue Virus Type 2",
abstract = "{\circledC} 2016 S. Karger AG, Basel. Background/Aims: The innate immune response is remarkably important for controlling infections. Information about the participation of antimicrobial peptides (AMPs) in response to dengue virus (DENV) is scarce. The aim of this study was to examine the AMP response to DENV-2 in human THP-1 cells and neutrophils. Methods: Protein and mRNA levels of two AMPs - hBD-1 and cathelicidin LL-37 - were assessed in DENV-infected macrophage-like THP-1 cells using qRT-PCR and indirect immunofluorescence. Also, mRNA levels of α-defensins (hDEFAs) and LL-37 were examined by qRT-PCR in human neutrophils taken from peripheral blood and treated with DENV-2. Results: mRNA expression of hBD-1 rose in THP-1 cells at 24-72 h, while protein expression increased later, from 48 to 72 h after infection. Cathelic idin LL-37 mRNA expression of DENV-infected THP-1 cells was observed at 6-48 h after infection, while protein levels increased importantly up to 72 h after infection. Regarding neutrophils, the mRNA expression of hDEFAs and LL-37 increased slightly at 2 and 5 h after the contact with DENV-2. Conclusion: THP-1 cells and human neutrophils strongly respond to DENV by producing AMPs: hBD-1 and LL-37 for the THP-1 cells and hDEFAs and LL-37 for neutrophils. However, the direct effect of these molecules on DENV particles remains unclear.",
author = "Casta{\~n}eda-S{\'a}nchez, {Jorge I.} and Dom{\'i}nguez-Mart{\'i}nez, {Diana Alhel{\'i}} and Nataly Olivar-Espinosa and Garc{\'i}a-P{\'e}rez, {Blanca Estela} and Loro{\~n}o-Pino, {Mar{\'i}a Alba} and Julieta Luna-Herrera and Salazar, {Ma Isabel}",
year = "2016",
month = "9",
day = "1",
doi = "10.1159/000446282",
language = "American English",
pages = "8--19",
journal = "Intervirology",
issn = "0300-5526",
publisher = "S. Karger AG",

}

Expression of Antimicrobial Peptides in Human Monocytic Cells and Neutrophils in Response to Dengue Virus Type 2. / Castañeda-Sánchez, Jorge I.; Domínguez-Martínez, Diana Alhelí; Olivar-Espinosa, Nataly; García-Pérez, Blanca Estela; Loroño-Pino, María Alba; Luna-Herrera, Julieta; Salazar, Ma Isabel.

In: Intervirology, 01.09.2016, p. 8-19.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of Antimicrobial Peptides in Human Monocytic Cells and Neutrophils in Response to Dengue Virus Type 2

AU - Castañeda-Sánchez, Jorge I.

AU - Domínguez-Martínez, Diana Alhelí

AU - Olivar-Espinosa, Nataly

AU - García-Pérez, Blanca Estela

AU - Loroño-Pino, María Alba

AU - Luna-Herrera, Julieta

AU - Salazar, Ma Isabel

PY - 2016/9/1

Y1 - 2016/9/1

N2 - © 2016 S. Karger AG, Basel. Background/Aims: The innate immune response is remarkably important for controlling infections. Information about the participation of antimicrobial peptides (AMPs) in response to dengue virus (DENV) is scarce. The aim of this study was to examine the AMP response to DENV-2 in human THP-1 cells and neutrophils. Methods: Protein and mRNA levels of two AMPs - hBD-1 and cathelicidin LL-37 - were assessed in DENV-infected macrophage-like THP-1 cells using qRT-PCR and indirect immunofluorescence. Also, mRNA levels of α-defensins (hDEFAs) and LL-37 were examined by qRT-PCR in human neutrophils taken from peripheral blood and treated with DENV-2. Results: mRNA expression of hBD-1 rose in THP-1 cells at 24-72 h, while protein expression increased later, from 48 to 72 h after infection. Cathelic idin LL-37 mRNA expression of DENV-infected THP-1 cells was observed at 6-48 h after infection, while protein levels increased importantly up to 72 h after infection. Regarding neutrophils, the mRNA expression of hDEFAs and LL-37 increased slightly at 2 and 5 h after the contact with DENV-2. Conclusion: THP-1 cells and human neutrophils strongly respond to DENV by producing AMPs: hBD-1 and LL-37 for the THP-1 cells and hDEFAs and LL-37 for neutrophils. However, the direct effect of these molecules on DENV particles remains unclear.

AB - © 2016 S. Karger AG, Basel. Background/Aims: The innate immune response is remarkably important for controlling infections. Information about the participation of antimicrobial peptides (AMPs) in response to dengue virus (DENV) is scarce. The aim of this study was to examine the AMP response to DENV-2 in human THP-1 cells and neutrophils. Methods: Protein and mRNA levels of two AMPs - hBD-1 and cathelicidin LL-37 - were assessed in DENV-infected macrophage-like THP-1 cells using qRT-PCR and indirect immunofluorescence. Also, mRNA levels of α-defensins (hDEFAs) and LL-37 were examined by qRT-PCR in human neutrophils taken from peripheral blood and treated with DENV-2. Results: mRNA expression of hBD-1 rose in THP-1 cells at 24-72 h, while protein expression increased later, from 48 to 72 h after infection. Cathelic idin LL-37 mRNA expression of DENV-infected THP-1 cells was observed at 6-48 h after infection, while protein levels increased importantly up to 72 h after infection. Regarding neutrophils, the mRNA expression of hDEFAs and LL-37 increased slightly at 2 and 5 h after the contact with DENV-2. Conclusion: THP-1 cells and human neutrophils strongly respond to DENV by producing AMPs: hBD-1 and LL-37 for the THP-1 cells and hDEFAs and LL-37 for neutrophils. However, the direct effect of these molecules on DENV particles remains unclear.

U2 - 10.1159/000446282

DO - 10.1159/000446282

M3 - Article

SP - 8

EP - 19

JO - Intervirology

JF - Intervirology

SN - 0300-5526

ER -