Expression of alternative NADH dehydrogenases (NDH-2) in the phytopathogenic fungus Ustilago maydis

Deyamira Matuz-Mares, Genaro Matus-Ortega, Christian Cárdenas-Monroy, Lucero Romero-Aguilar, Juan Carlos Villalobos-Rocha, Héctor Vázquez-Meza, Guadalupe Guerra-Sánchez, Antonio Peña-Díaz, Juan Pablo Pardo

    Research output: Contribution to journalArticlepeer-review

    3 Scopus citations

    Abstract

    Type 2 alternative NADH dehydrogenases (NDH-2) participate indirectly in the generation of the electrochemical proton gradient by transferring electrons from NADH and NADPH into the ubiquinone pool. Due to their structural simplicity, alternative NADH dehydrogenases have been proposed as useful tools for gene therapy of cells with defects in the respiratory complex I. In this work, we report the presence of three open reading frames, which correspond to NDH-2 genes in the genome of Ustilago maydis. These three genes were constitutively transcribed in cells cultured in YPD and minimal medium with glucose, ethanol, or lactate as carbon sources. Proteomic analysis showed that only two of the three NDH-2 were associated with isolated mitochondria in all culture media. Oxygen consumption by permeabilized cells using NADH or NADPH was different for each condition, opening the possibility of posttranslational regulation. We confirmed the presence of both external and internal NADH dehydrogenases, as well as an external NADPH dehydrogenase insensitive to calcium. Higher oxygen consumption rates were observed during the exponential growth phase, suggesting that the activity of NADH and NADPH dehydrogenases is coupled to the dynamics of cell growth.

    Original languageEnglish
    Pages (from-to)1267-1279
    Number of pages13
    JournalFEBS Open Bio
    Volume8
    Issue number8
    DOIs
    StatePublished - Aug 2018

    Keywords

    • alternative NAD(P)H dehydrogenases
    • corn smut fungi
    • electron transport chain
    • gene expression
    • NADH/NADPH quinone oxidoreductase
    • Ustilago maydis

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