Expression Changes in Metal-Resistance Genes in Microbacterium liquefaciens Under Nickel and Vanadium Exposure

Grisel Fierros-Romero, José A. Wrosek-Cabrera, Marlenne Gómez-Ramírez, Reynaldo C. Pless, A. M. Rivas-Castillo, Norma G. Rojas-Avelizapa

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Microbacterium liquefaciens MNSH2-PHGII-2 is a nickel–vanadium-resistant bacterium isolated from mine tailings located in Guanajuato, Mexico. In PHGII liquid media, M. liquefaciens has the ability to remove 29.5 ppm of Ni and 168.3 ppm of V. The present study reports, for the first time in M. liquefaciens, the presence of the genes nccA (Ni–Co–Cd resistance), hant (high-affinity nickel transporter), smtA, a metal-binding protein gene, and VAN2 (V resistance), which showed an increased expression under exposure to 200 ppm of Ni and 200 ppm of V during the logarithmic growth phase of the microorganism in PHGII liquid media. Data about the expression profile of genes conferring metal resistance to M. liquefaciens can improve the knowledge of those mechanisms involved in the processes of Ni–V resistance and probably in Ni–V removal processes. Based on our data, we can suggest that M. liquefaciens has the potential to be used in the biological treatment of toxic wastes with high Ni and V content.

Original languageEnglish
Pages (from-to)840-847
Number of pages8
JournalCurrent Microbiology
Volume74
Issue number7
DOIs
StatePublished - 1 Jul 2017
Externally publishedYes

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