TY - JOUR
T1 - Expression Changes in Metal-Resistance Genes in Microbacterium liquefaciens Under Nickel and Vanadium Exposure
AU - Fierros-Romero, Grisel
AU - Wrosek-Cabrera, José A.
AU - Gómez-Ramírez, Marlenne
AU - Pless, Reynaldo C.
AU - Rivas-Castillo, A. M.
AU - Rojas-Avelizapa, Norma G.
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media New York.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Microbacterium liquefaciens MNSH2-PHGII-2 is a nickel–vanadium-resistant bacterium isolated from mine tailings located in Guanajuato, Mexico. In PHGII liquid media, M. liquefaciens has the ability to remove 29.5 ppm of Ni and 168.3 ppm of V. The present study reports, for the first time in M. liquefaciens, the presence of the genes nccA (Ni–Co–Cd resistance), hant (high-affinity nickel transporter), smtA, a metal-binding protein gene, and VAN2 (V resistance), which showed an increased expression under exposure to 200 ppm of Ni and 200 ppm of V during the logarithmic growth phase of the microorganism in PHGII liquid media. Data about the expression profile of genes conferring metal resistance to M. liquefaciens can improve the knowledge of those mechanisms involved in the processes of Ni–V resistance and probably in Ni–V removal processes. Based on our data, we can suggest that M. liquefaciens has the potential to be used in the biological treatment of toxic wastes with high Ni and V content.
AB - Microbacterium liquefaciens MNSH2-PHGII-2 is a nickel–vanadium-resistant bacterium isolated from mine tailings located in Guanajuato, Mexico. In PHGII liquid media, M. liquefaciens has the ability to remove 29.5 ppm of Ni and 168.3 ppm of V. The present study reports, for the first time in M. liquefaciens, the presence of the genes nccA (Ni–Co–Cd resistance), hant (high-affinity nickel transporter), smtA, a metal-binding protein gene, and VAN2 (V resistance), which showed an increased expression under exposure to 200 ppm of Ni and 200 ppm of V during the logarithmic growth phase of the microorganism in PHGII liquid media. Data about the expression profile of genes conferring metal resistance to M. liquefaciens can improve the knowledge of those mechanisms involved in the processes of Ni–V resistance and probably in Ni–V removal processes. Based on our data, we can suggest that M. liquefaciens has the potential to be used in the biological treatment of toxic wastes with high Ni and V content.
UR - http://www.scopus.com/inward/record.url?scp=85018285933&partnerID=8YFLogxK
U2 - 10.1007/s00284-017-1252-8
DO - 10.1007/s00284-017-1252-8
M3 - Artículo
C2 - 28447152
SN - 0343-8651
VL - 74
SP - 840
EP - 847
JO - Current Microbiology
JF - Current Microbiology
IS - 7
ER -