TY - JOUR
T1 - Expression and distribution of dopamine transporter in cardiac tissues of the guinea pig
AU - Palomar, Alejandro Reynoso
AU - Larios, Berenice Navarrete
AU - De Sánchez, Victoria Chagoya
AU - Pérez, Lidia Martínez
AU - De La Cruz López, Fidel
AU - Flores, Gonzalo
AU - De Jesus Gomez-Villalobos, Maria
N1 - Funding Information:
Acknowledgments This study was supported by grants from VIEP-BUAP (GOVM-SAL08-I). We are grateful to Ruth Giles for their technical assistance, and we thank Dr. Ellis Glazier for editing this English-language text.
PY - 2011/3
Y1 - 2011/3
N2 - Dopamine transporter (DAT) is a membrane protein that it is a marker for dopaminergic neurons. In the present work, throught Western blot and autoradiographic studies with a selective ligand for DAT ([ 3H] WIN-35428) and noradrenaline transporter (NET) ([ 3H] Nisoxetine), we search the expression and distribution of DAT in comparison with NET, in cardiac tissue of guinea pig in order to support the presence of dopaminergic nerve cells into the heart. Expression of DAT, and NET were evidenced by a bands of 75 and 54 kDa, respectively in the heart. Binding for DAT and NET were found in the four cardiac chambers. However, DAT show heterogeneous distribution with binding in right atria and in both ventricles, whereas NET show homogenous distribution in the four cardiac chambers. The results show the expression of DAT in cardiac tissues with a different distribution compared with NET, being an evidence for the presence of dopaminergic nerve cells into the heart.
AB - Dopamine transporter (DAT) is a membrane protein that it is a marker for dopaminergic neurons. In the present work, throught Western blot and autoradiographic studies with a selective ligand for DAT ([ 3H] WIN-35428) and noradrenaline transporter (NET) ([ 3H] Nisoxetine), we search the expression and distribution of DAT in comparison with NET, in cardiac tissue of guinea pig in order to support the presence of dopaminergic nerve cells into the heart. Expression of DAT, and NET were evidenced by a bands of 75 and 54 kDa, respectively in the heart. Binding for DAT and NET were found in the four cardiac chambers. However, DAT show heterogeneous distribution with binding in right atria and in both ventricles, whereas NET show homogenous distribution in the four cardiac chambers. The results show the expression of DAT in cardiac tissues with a different distribution compared with NET, being an evidence for the presence of dopaminergic nerve cells into the heart.
KW - Autoradiographic studies
KW - Dopamine transporter
KW - Guinea pig heart
KW - Noradrenaline transporter
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=79954419344&partnerID=8YFLogxK
U2 - 10.1007/s11064-010-0344-7
DO - 10.1007/s11064-010-0344-7
M3 - Artículo
SN - 0364-3190
VL - 36
SP - 399
EP - 405
JO - Neurochemical Research
JF - Neurochemical Research
IS - 3
ER -