Expression Analysis of Ni- and V-Associated Resistance Genes in a Bacillus megaterium Strain Isolated from a Mining Site

Grisel Fierros Romero, Andrea Rivas Castillo, Marlenne Gómez Ramírez, Reynaldo Pless, Norma Rojas Avelizapa

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    Abstract

    © 2016, Springer Science+Business Media New York. Bacillus megaterium strain MNSH1-9K-1 was isolated from a mining site in Guanajuato, Mexico. This B. megaterium strain presented the ability to remove Ni and V from a spent catalyst. Also, its associated metal resistance genes nccA, hant, VAN2, and smtAB were previously identified by a PCR approach. The present study reports for the first time, in B. megaterium, the changes in the expression of the genes nccA (Ni–Co–Cd resistance); hant (high-affinity nickel transporter); smtAB, a metal-binding protein gene; and VAN2 (V resistance) after exposure to 200 ppm of Ni and 200 ppm of V during the stationary phase of the microorganism in PHGII liquid media. The data presented here may contribute to the knowledge of the genes involved in the Ni and V resistances of B. megaterium, and the possible pathways implicated in the Ni–V removal processes, which may be potentiated for the biological treatment of high metal content residues.
    Original languageAmerican English
    Pages (from-to)165-171
    Number of pages147
    JournalCurrent Microbiology
    DOIs
    StatePublished - 1 Aug 2016

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