TY - JOUR
T1 - Exposure to bisphenol A in young adult mice does not alter ovulation but does alter the fertilization ability of oocytes
AU - Moore-Ambriz, Teresita Rocio
AU - Acuña-Hernández, Deyanira Guadalupe
AU - Ramos-Robles, Brenda
AU - Sánchez-Gutiérrez, Manuel
AU - Santacruz-Márquez, Ramsés
AU - Sierra-Santoyo, Adolfo
AU - Piña-Guzmán, Belem
AU - Shibayama, Mineko
AU - Hernández-Ochoa, Isabel
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/12/15
Y1 - 2015/12/15
N2 - Follicle growth culminates in ovulation, which allows for the expulsion of fertilizable oocytes and the formation of corpora lutea. Bisphenol A (BPA) is present in many consumer products, and it has been suggested that BPA impairs ovulation; however, the underlying mechanisms are unknown. Therefore, this study first evaluated whether BPA alters ovulation by affecting folliculogenesis, the number of corpora lutea or eggs shed to the oviduct, ovarian gonadotropin responsiveness, hormone levels, and estrous cyclicity. Because it has been suggested (but not directly confirmed) that BPA exerts toxic effects on the fertilization ability of oocytes, a second aim was to evaluate whether BPA impacts the oocyte fertilization rate using an in vitro fertilization assay and mating. The possible effects on early zygote development were also examined. Young adult female C57BL/6J mice (39 days old) were orally dosed with corn oil (vehicle) or 50 μg/kg bw/day BPA for a period encompassing the first three reproductive cycles (12-15 days). BPA exposure did not alter any parameters related to ovulation. Moreover, BPA exposure reduced the percentage of fertilized oocytes after either in vitro fertilization or mating, but it did not alter the zygotic stages. The data indicate that exposure to the reference dose of BPA does not impact ovulation but that it does influence the oocyte quality in terms of its fertilization ability.
AB - Follicle growth culminates in ovulation, which allows for the expulsion of fertilizable oocytes and the formation of corpora lutea. Bisphenol A (BPA) is present in many consumer products, and it has been suggested that BPA impairs ovulation; however, the underlying mechanisms are unknown. Therefore, this study first evaluated whether BPA alters ovulation by affecting folliculogenesis, the number of corpora lutea or eggs shed to the oviduct, ovarian gonadotropin responsiveness, hormone levels, and estrous cyclicity. Because it has been suggested (but not directly confirmed) that BPA exerts toxic effects on the fertilization ability of oocytes, a second aim was to evaluate whether BPA impacts the oocyte fertilization rate using an in vitro fertilization assay and mating. The possible effects on early zygote development were also examined. Young adult female C57BL/6J mice (39 days old) were orally dosed with corn oil (vehicle) or 50 μg/kg bw/day BPA for a period encompassing the first three reproductive cycles (12-15 days). BPA exposure did not alter any parameters related to ovulation. Moreover, BPA exposure reduced the percentage of fertilized oocytes after either in vitro fertilization or mating, but it did not alter the zygotic stages. The data indicate that exposure to the reference dose of BPA does not impact ovulation but that it does influence the oocyte quality in terms of its fertilization ability.
KW - Bisphenol A
KW - In vitro fertilization
KW - In vivo fertilization
KW - Oocyte
KW - Ovulation
UR - http://www.scopus.com/inward/record.url?scp=84947760985&partnerID=8YFLogxK
U2 - 10.1016/j.taap.2015.10.010
DO - 10.1016/j.taap.2015.10.010
M3 - Artículo
SN - 0041-008X
VL - 289
SP - 507
EP - 514
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
IS - 3
ER -