TY - JOUR
T1 - Exposure of fluoride with streptozotocin-induced diabetes aggravates testicular damage and spermatozoa parameters in mice
AU - Sánchez-Gutiérrez, Manuel
AU - Martínez-Loredo, Evelia
AU - Madrigal-Santillán, Eduardo Osiris
AU - Betanzos-Cabrera, Gabriel
AU - Hernández-Zavala, Araceli
AU - Mojica-Villegas, María Angélica
AU - Izquierdo-Vega, Jeannett Alejandra
N1 - Publisher Copyright:
© 2019 Sánchez-Gutiérrez et al.
PY - 2019
Y1 - 2019
N2 - Diabetes mellitus is the most common chronic disease worldwide that causes numerous complications, including male infertility. The prevalence of DM is 451 million people and estimated that would increase to 693 million in 2045. Fluorosis caused by drinking water contaminated with inorganic fluoride is a public health problem in many areas around the world. Previous studies have shown that fluoride exposure damages the male reproductive function. This study aimed to evaluate the fluoride sub-chronic exposure on the spermatozoa function in streptozotocin (STZ)-induced diabetic mice. After confirming diabetes by measuring blood glucose levels, the male mice received 45.2 ppm of fluoride added or deionized water. We evaluated several parameters in diabetic mice exposed to fluoride: standard quality analysis, the mitochondrial transmembrane potential (ψm), the caspase activity in spermatozoa, urinary fluoride excretion, and histological evaluation in the testes. After 60 days of fluoride-exposure, diabetic mice, significantly decreased sperm quality (motility, viability, and concentration). Spermatozoa from fluoride-exposure in diabetic mice presented a significant decrease in ψm and a significant increase in activity caspase 3/7. Urinary fluoride excretion was decreased in diabetic mice exposed to fluoride. Subchronic fluoride exposure of mice with STZ-induced diabetes aggravated testicular damage and the spermatozoa function.
AB - Diabetes mellitus is the most common chronic disease worldwide that causes numerous complications, including male infertility. The prevalence of DM is 451 million people and estimated that would increase to 693 million in 2045. Fluorosis caused by drinking water contaminated with inorganic fluoride is a public health problem in many areas around the world. Previous studies have shown that fluoride exposure damages the male reproductive function. This study aimed to evaluate the fluoride sub-chronic exposure on the spermatozoa function in streptozotocin (STZ)-induced diabetic mice. After confirming diabetes by measuring blood glucose levels, the male mice received 45.2 ppm of fluoride added or deionized water. We evaluated several parameters in diabetic mice exposed to fluoride: standard quality analysis, the mitochondrial transmembrane potential (ψm), the caspase activity in spermatozoa, urinary fluoride excretion, and histological evaluation in the testes. After 60 days of fluoride-exposure, diabetic mice, significantly decreased sperm quality (motility, viability, and concentration). Spermatozoa from fluoride-exposure in diabetic mice presented a significant decrease in ψm and a significant increase in activity caspase 3/7. Urinary fluoride excretion was decreased in diabetic mice exposed to fluoride. Subchronic fluoride exposure of mice with STZ-induced diabetes aggravated testicular damage and the spermatozoa function.
UR - http://www.scopus.com/inward/record.url?scp=85077014982&partnerID=8YFLogxK
U2 - 10.1155/2019/5269380
DO - 10.1155/2019/5269380
M3 - Artículo
C2 - 31885555
AN - SCOPUS:85077014982
SN - 1687-8191
VL - 2019
JO - Journal of Toxicology
JF - Journal of Toxicology
M1 - 5269380
ER -