Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility

Francisco Jimeńez-Trejo, Miguel Tapia-Rodriǵuez, Marco Cerboń, Donald M. Kuhn, Gabriel Manjarrez-Gutieŕrez, C. Adriana Mendoza-Rodríguez, Ofir Picazo

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22 Citations (Scopus)

Abstract

Serotonin (5-hydroxytryptamine; C10H12N2O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAOA, 5-HT1B, 5-HT3, and 5HTT; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAOA, 5-HT1B, 5-HT2A, 5-HT3, 5-HTT, and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically. © 2012 Society for Reproduction and Fertility.
Original languageAmerican English
Pages (from-to)677-685
Number of pages9
JournalReproduction
DOIs
StatePublished - 1 Dec 2012

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Tyrosine
Spermatozoa
Serotonin
Phosphorylation
Proteins
Tryptophan Hydroxylase
Sperm Motility
Western Blotting
Semen Analysis
Leydig Cells
Epididymis
Human Activities
Cricetinae
Reproduction
Fertility
Testis
Immunohistochemistry
High Pressure Liquid Chromatography
Pharmacology

Cite this

Jimeńez-Trejo, F., Tapia-Rodriǵuez, M., Cerboń, M., Kuhn, D. M., Manjarrez-Gutieŕrez, G., Mendoza-Rodríguez, C. A., & Picazo, O. (2012). Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility. Reproduction, 677-685. https://doi.org/10.1530/REP-12-0145
Jimeńez-Trejo, Francisco ; Tapia-Rodriǵuez, Miguel ; Cerboń, Marco ; Kuhn, Donald M. ; Manjarrez-Gutieŕrez, Gabriel ; Mendoza-Rodríguez, C. Adriana ; Picazo, Ofir. / Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility. In: Reproduction. 2012 ; pp. 677-685.
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abstract = "Serotonin (5-hydroxytryptamine; C10H12N2O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAOA, 5-HT1B, 5-HT3, and 5HTT; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAOA, 5-HT1B, 5-HT2A, 5-HT3, 5-HTT, and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically. {\circledC} 2012 Society for Reproduction and Fertility.",
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Jimeńez-Trejo, F, Tapia-Rodriǵuez, M, Cerboń, M, Kuhn, DM, Manjarrez-Gutieŕrez, G, Mendoza-Rodríguez, CA & Picazo, O 2012, 'Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility', Reproduction, pp. 677-685. https://doi.org/10.1530/REP-12-0145

Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility. / Jimeńez-Trejo, Francisco; Tapia-Rodriǵuez, Miguel; Cerboń, Marco; Kuhn, Donald M.; Manjarrez-Gutieŕrez, Gabriel; Mendoza-Rodríguez, C. Adriana; Picazo, Ofir.

In: Reproduction, 01.12.2012, p. 677-685.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility

AU - Jimeńez-Trejo, Francisco

AU - Tapia-Rodriǵuez, Miguel

AU - Cerboń, Marco

AU - Kuhn, Donald M.

AU - Manjarrez-Gutieŕrez, Gabriel

AU - Mendoza-Rodríguez, C. Adriana

AU - Picazo, Ofir

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Serotonin (5-hydroxytryptamine; C10H12N2O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAOA, 5-HT1B, 5-HT3, and 5HTT; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAOA, 5-HT1B, 5-HT2A, 5-HT3, 5-HTT, and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically. © 2012 Society for Reproduction and Fertility.

AB - Serotonin (5-hydroxytryptamine; C10H12N2O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAOA, 5-HT1B, 5-HT3, and 5HTT; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAOA, 5-HT1B, 5-HT2A, 5-HT3, 5-HTT, and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically. © 2012 Society for Reproduction and Fertility.

U2 - 10.1530/REP-12-0145

DO - 10.1530/REP-12-0145

M3 - Article

SP - 677

EP - 685

JO - Reproduction

JF - Reproduction

SN - 1470-1626

ER -

Jimeńez-Trejo F, Tapia-Rodriǵuez M, Cerboń M, Kuhn DM, Manjarrez-Gutieŕrez G, Mendoza-Rodríguez CA et al. Evidence of 5-HT components in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility. Reproduction. 2012 Dec 1;677-685. https://doi.org/10.1530/REP-12-0145