Evaluation of the sensitivity to chlorhexidine, voriconazole and itraconazole of T4 genotype Acanthamoeba isolated from Mexico

Dolores Hernández-Martínez, María Reyes-Batlle, Ismael Castelan-Ramírez, Perla Hernández-Olmos, Virginia Vanzzini-Zago, Elizabeth Ramírez-Flores, Inés Sifaoui, José E. Piñero, Jacob Lorenzo-Morales, Maritza Omaña-Molina

Research output: Contribution to journalArticleResearchpeer-review

Abstract

© 2019 Free-living amoebae of the genus Acanthamoeba are the etiological agents of cutaneous lesions, granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK), which are chronic infections with poor prognosis if not diagnosed promptly. Currently, there is no optimal therapeutic scheme to eradicate the pathologies these protozoa cause. In this study we report the morphological and molecular identification of three species of the genus Acanthamoeba, belonging to T4 group; A. polyphaga isolated from the corneal ulcer of a patient sample of AK case; A. castellanii isolated from the contact lens of an AK patient and A. palestinensis obtained from a soil sample. The in vitro activity of chlorhexidine, itraconazole and voriconazole drugs against trophic stage was also evaluated through a colorimetric assay based on the oxidation-reduction of alamar blue. The strains in the study were sensitive to the evaluated drugs; although when determining the 50% inhibitory concentration (IC 50 ) statistically significant differences were observed. A. castellanii showed to be highly sensitive to voriconazole (0.66 ± 0.13 μM) but the least sensitive to chlorhexidine and itraconazole (8.61 ± 1.63 and 20.14 ± 4.93 μM, respectively), A. palestinensis showed the highest sensitivity to itraconazole (0.502 ± 0.11 μM) and A. polyphaga expressed moderate sensitivity to chlorhexidine and itraconazole and lower sensitivity to voriconazole (10.10 ± 2.21 μM). These results showed that species of the genus Acanthamoeba express different sensitivity to the tested drugs, which could explain the problems surrounding the establishment of a treatment of choice in the infections caused by these amoebae. We consider that although chlorhexidine and itraconazole show good activity on these amoebae and have been used in cases of AK in Mexico with acceptable results, voriconazole should be considered as the first therapeutic option of future Acanthamoeba infections that will be diagnosed in our country.
Original languageAmerican English
Pages (from-to)29-35
Number of pages25
JournalExperimental Parasitology
DOIs
StatePublished - 1 Feb 2019
Externally publishedYes

Fingerprint

Acanthamoeba
Chlorhexidine
Itraconazole
Mexico
Keratitis
Genotype
Amoeba
Infection
Pharmaceutical Preparations
Corneal Ulcer
Contact Lenses
Encephalitis
Inhibitory Concentration 50
Oxidation-Reduction
Soil
Therapeutics
Voriconazole
Pathology
Skin

Cite this

Hernández-Martínez, Dolores ; Reyes-Batlle, María ; Castelan-Ramírez, Ismael ; Hernández-Olmos, Perla ; Vanzzini-Zago, Virginia ; Ramírez-Flores, Elizabeth ; Sifaoui, Inés ; Piñero, José E. ; Lorenzo-Morales, Jacob ; Omaña-Molina, Maritza. / Evaluation of the sensitivity to chlorhexidine, voriconazole and itraconazole of T4 genotype Acanthamoeba isolated from Mexico. In: Experimental Parasitology. 2019 ; pp. 29-35.
@article{26ab65b06f5547c0a5dca5aafc159c7e,
title = "Evaluation of the sensitivity to chlorhexidine, voriconazole and itraconazole of T4 genotype Acanthamoeba isolated from Mexico",
abstract = "{\circledC} 2019 Free-living amoebae of the genus Acanthamoeba are the etiological agents of cutaneous lesions, granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK), which are chronic infections with poor prognosis if not diagnosed promptly. Currently, there is no optimal therapeutic scheme to eradicate the pathologies these protozoa cause. In this study we report the morphological and molecular identification of three species of the genus Acanthamoeba, belonging to T4 group; A. polyphaga isolated from the corneal ulcer of a patient sample of AK case; A. castellanii isolated from the contact lens of an AK patient and A. palestinensis obtained from a soil sample. The in vitro activity of chlorhexidine, itraconazole and voriconazole drugs against trophic stage was also evaluated through a colorimetric assay based on the oxidation-reduction of alamar blue. The strains in the study were sensitive to the evaluated drugs; although when determining the 50{\%} inhibitory concentration (IC 50 ) statistically significant differences were observed. A. castellanii showed to be highly sensitive to voriconazole (0.66 ± 0.13 μM) but the least sensitive to chlorhexidine and itraconazole (8.61 ± 1.63 and 20.14 ± 4.93 μM, respectively), A. palestinensis showed the highest sensitivity to itraconazole (0.502 ± 0.11 μM) and A. polyphaga expressed moderate sensitivity to chlorhexidine and itraconazole and lower sensitivity to voriconazole (10.10 ± 2.21 μM). These results showed that species of the genus Acanthamoeba express different sensitivity to the tested drugs, which could explain the problems surrounding the establishment of a treatment of choice in the infections caused by these amoebae. We consider that although chlorhexidine and itraconazole show good activity on these amoebae and have been used in cases of AK in Mexico with acceptable results, voriconazole should be considered as the first therapeutic option of future Acanthamoeba infections that will be diagnosed in our country.",
author = "Dolores Hern{\'a}ndez-Mart{\'i}nez and Mar{\'i}a Reyes-Batlle and Ismael Castelan-Ram{\'i}rez and Perla Hern{\'a}ndez-Olmos and Virginia Vanzzini-Zago and Elizabeth Ram{\'i}rez-Flores and In{\'e}s Sifaoui and Pi{\~n}ero, {Jos{\'e} E.} and Jacob Lorenzo-Morales and Maritza Oma{\~n}a-Molina",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.exppara.2019.01.006",
language = "American English",
pages = "29--35",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",

}

Hernández-Martínez, D, Reyes-Batlle, M, Castelan-Ramírez, I, Hernández-Olmos, P, Vanzzini-Zago, V, Ramírez-Flores, E, Sifaoui, I, Piñero, JE, Lorenzo-Morales, J & Omaña-Molina, M 2019, 'Evaluation of the sensitivity to chlorhexidine, voriconazole and itraconazole of T4 genotype Acanthamoeba isolated from Mexico', Experimental Parasitology, pp. 29-35. https://doi.org/10.1016/j.exppara.2019.01.006

Evaluation of the sensitivity to chlorhexidine, voriconazole and itraconazole of T4 genotype Acanthamoeba isolated from Mexico. / Hernández-Martínez, Dolores; Reyes-Batlle, María; Castelan-Ramírez, Ismael; Hernández-Olmos, Perla; Vanzzini-Zago, Virginia; Ramírez-Flores, Elizabeth; Sifaoui, Inés; Piñero, José E.; Lorenzo-Morales, Jacob; Omaña-Molina, Maritza.

In: Experimental Parasitology, 01.02.2019, p. 29-35.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Evaluation of the sensitivity to chlorhexidine, voriconazole and itraconazole of T4 genotype Acanthamoeba isolated from Mexico

AU - Hernández-Martínez, Dolores

AU - Reyes-Batlle, María

AU - Castelan-Ramírez, Ismael

AU - Hernández-Olmos, Perla

AU - Vanzzini-Zago, Virginia

AU - Ramírez-Flores, Elizabeth

AU - Sifaoui, Inés

AU - Piñero, José E.

AU - Lorenzo-Morales, Jacob

AU - Omaña-Molina, Maritza

PY - 2019/2/1

Y1 - 2019/2/1

N2 - © 2019 Free-living amoebae of the genus Acanthamoeba are the etiological agents of cutaneous lesions, granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK), which are chronic infections with poor prognosis if not diagnosed promptly. Currently, there is no optimal therapeutic scheme to eradicate the pathologies these protozoa cause. In this study we report the morphological and molecular identification of three species of the genus Acanthamoeba, belonging to T4 group; A. polyphaga isolated from the corneal ulcer of a patient sample of AK case; A. castellanii isolated from the contact lens of an AK patient and A. palestinensis obtained from a soil sample. The in vitro activity of chlorhexidine, itraconazole and voriconazole drugs against trophic stage was also evaluated through a colorimetric assay based on the oxidation-reduction of alamar blue. The strains in the study were sensitive to the evaluated drugs; although when determining the 50% inhibitory concentration (IC 50 ) statistically significant differences were observed. A. castellanii showed to be highly sensitive to voriconazole (0.66 ± 0.13 μM) but the least sensitive to chlorhexidine and itraconazole (8.61 ± 1.63 and 20.14 ± 4.93 μM, respectively), A. palestinensis showed the highest sensitivity to itraconazole (0.502 ± 0.11 μM) and A. polyphaga expressed moderate sensitivity to chlorhexidine and itraconazole and lower sensitivity to voriconazole (10.10 ± 2.21 μM). These results showed that species of the genus Acanthamoeba express different sensitivity to the tested drugs, which could explain the problems surrounding the establishment of a treatment of choice in the infections caused by these amoebae. We consider that although chlorhexidine and itraconazole show good activity on these amoebae and have been used in cases of AK in Mexico with acceptable results, voriconazole should be considered as the first therapeutic option of future Acanthamoeba infections that will be diagnosed in our country.

AB - © 2019 Free-living amoebae of the genus Acanthamoeba are the etiological agents of cutaneous lesions, granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK), which are chronic infections with poor prognosis if not diagnosed promptly. Currently, there is no optimal therapeutic scheme to eradicate the pathologies these protozoa cause. In this study we report the morphological and molecular identification of three species of the genus Acanthamoeba, belonging to T4 group; A. polyphaga isolated from the corneal ulcer of a patient sample of AK case; A. castellanii isolated from the contact lens of an AK patient and A. palestinensis obtained from a soil sample. The in vitro activity of chlorhexidine, itraconazole and voriconazole drugs against trophic stage was also evaluated through a colorimetric assay based on the oxidation-reduction of alamar blue. The strains in the study were sensitive to the evaluated drugs; although when determining the 50% inhibitory concentration (IC 50 ) statistically significant differences were observed. A. castellanii showed to be highly sensitive to voriconazole (0.66 ± 0.13 μM) but the least sensitive to chlorhexidine and itraconazole (8.61 ± 1.63 and 20.14 ± 4.93 μM, respectively), A. palestinensis showed the highest sensitivity to itraconazole (0.502 ± 0.11 μM) and A. polyphaga expressed moderate sensitivity to chlorhexidine and itraconazole and lower sensitivity to voriconazole (10.10 ± 2.21 μM). These results showed that species of the genus Acanthamoeba express different sensitivity to the tested drugs, which could explain the problems surrounding the establishment of a treatment of choice in the infections caused by these amoebae. We consider that although chlorhexidine and itraconazole show good activity on these amoebae and have been used in cases of AK in Mexico with acceptable results, voriconazole should be considered as the first therapeutic option of future Acanthamoeba infections that will be diagnosed in our country.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85060123765&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85060123765&origin=inward

U2 - 10.1016/j.exppara.2019.01.006

DO - 10.1016/j.exppara.2019.01.006

M3 - Article

SP - 29

EP - 35

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

ER -