Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts

Karla Stephanie Martinez-Elizalde, Manuel Jimenez-Estrada, Cesar Mateo Flores, Luis Barbo Hernandez, Rocio Rosas-Lopez, Angel Duran-Diaz, Oscar J. Nieto-Yañez, Elizabeth Barbosa, Marco Aurelio Rodriguez-Monroy, Margarita Canales-Martinez

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Abstract

© Martinez-Elizalde et al.; licensee BioMed Central. Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.
Original languageAmerican English
JournalBMC Complementary and Alternative Medicine
DOIs
StatePublished - 21 Mar 2015

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Methanol
Fruit
Interleukin-1
Macrophages
Stomach Diseases
Vibrio cholerae
Palmitic Acid
National Cancer Institute (U.S.)
Poisons
Lethal Dose 50
Gram-Positive Bacteria
Hexanes
Linoleic Acid
Gram-Negative Bacteria
Bacterial Infections
Citric Acid
Free Radicals
Chromatography
Diarrhea
Anti-Inflammatory Agents

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Martinez-Elizalde, K. S., Jimenez-Estrada, M., Flores, C. M., Hernandez, L. B., Rosas-Lopez, R., Duran-Diaz, A., ... Canales-Martinez, M. (2015). Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts. BMC Complementary and Alternative Medicine. https://doi.org/10.1186/s12906-015-0602-y
Martinez-Elizalde, Karla Stephanie ; Jimenez-Estrada, Manuel ; Flores, Cesar Mateo ; Hernandez, Luis Barbo ; Rosas-Lopez, Rocio ; Duran-Diaz, Angel ; Nieto-Yañez, Oscar J. ; Barbosa, Elizabeth ; Rodriguez-Monroy, Marco Aurelio ; Canales-Martinez, Margarita. / Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts. In: BMC Complementary and Alternative Medicine. 2015.
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abstract = "{\circledC} Martinez-Elizalde et al.; licensee BioMed Central. Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.",
author = "Martinez-Elizalde, {Karla Stephanie} and Manuel Jimenez-Estrada and Flores, {Cesar Mateo} and Hernandez, {Luis Barbo} and Rocio Rosas-Lopez and Angel Duran-Diaz and Nieto-Ya{\~n}ez, {Oscar J.} and Elizabeth Barbosa and Rodriguez-Monroy, {Marco Aurelio} and Margarita Canales-Martinez",
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Martinez-Elizalde, KS, Jimenez-Estrada, M, Flores, CM, Hernandez, LB, Rosas-Lopez, R, Duran-Diaz, A, Nieto-Yañez, OJ, Barbosa, E, Rodriguez-Monroy, MA & Canales-Martinez, M 2015, 'Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts', BMC Complementary and Alternative Medicine. https://doi.org/10.1186/s12906-015-0602-y

Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts. / Martinez-Elizalde, Karla Stephanie; Jimenez-Estrada, Manuel; Flores, Cesar Mateo; Hernandez, Luis Barbo; Rosas-Lopez, Rocio; Duran-Diaz, Angel; Nieto-Yañez, Oscar J.; Barbosa, Elizabeth; Rodriguez-Monroy, Marco Aurelio; Canales-Martinez, Margarita.

In: BMC Complementary and Alternative Medicine, 21.03.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts

AU - Martinez-Elizalde, Karla Stephanie

AU - Jimenez-Estrada, Manuel

AU - Flores, Cesar Mateo

AU - Hernandez, Luis Barbo

AU - Rosas-Lopez, Rocio

AU - Duran-Diaz, Angel

AU - Nieto-Yañez, Oscar J.

AU - Barbosa, Elizabeth

AU - Rodriguez-Monroy, Marco Aurelio

AU - Canales-Martinez, Margarita

PY - 2015/3/21

Y1 - 2015/3/21

N2 - © Martinez-Elizalde et al.; licensee BioMed Central. Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.

AB - © Martinez-Elizalde et al.; licensee BioMed Central. Background: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. Methods: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. Results: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC=4 mg/mL) and Vibrio cholera (MIC=4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50=69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50=22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. Conclusions: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.

U2 - 10.1186/s12906-015-0602-y

DO - 10.1186/s12906-015-0602-y

M3 - Article

JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

SN - 1472-6882

ER -

Martinez-Elizalde KS, Jimenez-Estrada M, Flores CM, Hernandez LB, Rosas-Lopez R, Duran-Diaz A et al. Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts. BMC Complementary and Alternative Medicine. 2015 Mar 21. https://doi.org/10.1186/s12906-015-0602-y