Evaluation of morphological properties of Solanum chrysotrichum cell cultures in a shake flask and fermenter and rheological properties of broths

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Abstract

Solanum chrysotrichum cells were grown in a shake flask and a stirred tank bioreactor. Their growth kinetics and Theological and morphological behaviors were evaluated. The specific growth rate obtained in both systems was similar (0.09 day-1), but biomass yield achieved in shake flasks was 60 % higher than that in the fermentor. Culture broths exhibited non-Newtonian and shear-thinning characteristics, which can be simulated by the Ostwald de Waele model (power law). Results show that the pseudoplasticity of culture broths was determined by the biomass concentration and aggregate size distribution, while the aggregate roundness, measured as the elliptical form factor, was similar in both systems. Morphological analysis indicated that 100% of the cell aggregates from the fermentor were <0.25 mm2, and their size distribution remained constant during cultivation. The cultures grown in shake flasks presented a tendency to increase their aggregate size as a consequence of cell growth. The reduction of the size of S. chrysotrichum aggregates during the scale-up had an important influence on flow index behavior and consistency coefficient values.
Original languageAmerican English
Pages (from-to)153-158
Number of pages137
JournalFood Technology and Biotechnology
StatePublished - 1 Jul 2004

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Solanum chrysotrichum
Fermenters
Solanum
fermenters
Bioreactors
cell aggregates
rheological properties
Cell culture
cell culture
Cell Culture Techniques
Biomass
Growth
Shear thinning
Growth kinetics
biomass
Cell growth
bioreactors
specific growth rate
shear stress
cell growth

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title = "Evaluation of morphological properties of Solanum chrysotrichum cell cultures in a shake flask and fermenter and rheological properties of broths",
abstract = "Solanum chrysotrichum cells were grown in a shake flask and a stirred tank bioreactor. Their growth kinetics and Theological and morphological behaviors were evaluated. The specific growth rate obtained in both systems was similar (0.09 day-1), but biomass yield achieved in shake flasks was 60 {\%} higher than that in the fermentor. Culture broths exhibited non-Newtonian and shear-thinning characteristics, which can be simulated by the Ostwald de Waele model (power law). Results show that the pseudoplasticity of culture broths was determined by the biomass concentration and aggregate size distribution, while the aggregate roundness, measured as the elliptical form factor, was similar in both systems. Morphological analysis indicated that 100{\%} of the cell aggregates from the fermentor were <0.25 mm2, and their size distribution remained constant during cultivation. The cultures grown in shake flasks presented a tendency to increase their aggregate size as a consequence of cell growth. The reduction of the size of S. chrysotrichum aggregates during the scale-up had an important influence on flow index behavior and consistency coefficient values.",
author = "Mario Rodr{\'i}guez-Monroy and Trejo-Espino, {Jos{\'e} Luis} and Antonio Jim{\'e}nez-Aparicio and {De La Luz Morante}, Ma and Villarreal, {Ma Luisa} and Gabriela Trejo-Tapia",
year = "2004",
month = "7",
day = "1",
language = "American English",
pages = "153--158",
journal = "Food Technology and Biotechnology",
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publisher = "University of Zagreb",

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TY - JOUR

T1 - Evaluation of morphological properties of Solanum chrysotrichum cell cultures in a shake flask and fermenter and rheological properties of broths

AU - Rodríguez-Monroy, Mario

AU - Trejo-Espino, José Luis

AU - Jiménez-Aparicio, Antonio

AU - De La Luz Morante, Ma

AU - Villarreal, Ma Luisa

AU - Trejo-Tapia, Gabriela

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Solanum chrysotrichum cells were grown in a shake flask and a stirred tank bioreactor. Their growth kinetics and Theological and morphological behaviors were evaluated. The specific growth rate obtained in both systems was similar (0.09 day-1), but biomass yield achieved in shake flasks was 60 % higher than that in the fermentor. Culture broths exhibited non-Newtonian and shear-thinning characteristics, which can be simulated by the Ostwald de Waele model (power law). Results show that the pseudoplasticity of culture broths was determined by the biomass concentration and aggregate size distribution, while the aggregate roundness, measured as the elliptical form factor, was similar in both systems. Morphological analysis indicated that 100% of the cell aggregates from the fermentor were <0.25 mm2, and their size distribution remained constant during cultivation. The cultures grown in shake flasks presented a tendency to increase their aggregate size as a consequence of cell growth. The reduction of the size of S. chrysotrichum aggregates during the scale-up had an important influence on flow index behavior and consistency coefficient values.

AB - Solanum chrysotrichum cells were grown in a shake flask and a stirred tank bioreactor. Their growth kinetics and Theological and morphological behaviors were evaluated. The specific growth rate obtained in both systems was similar (0.09 day-1), but biomass yield achieved in shake flasks was 60 % higher than that in the fermentor. Culture broths exhibited non-Newtonian and shear-thinning characteristics, which can be simulated by the Ostwald de Waele model (power law). Results show that the pseudoplasticity of culture broths was determined by the biomass concentration and aggregate size distribution, while the aggregate roundness, measured as the elliptical form factor, was similar in both systems. Morphological analysis indicated that 100% of the cell aggregates from the fermentor were <0.25 mm2, and their size distribution remained constant during cultivation. The cultures grown in shake flasks presented a tendency to increase their aggregate size as a consequence of cell growth. The reduction of the size of S. chrysotrichum aggregates during the scale-up had an important influence on flow index behavior and consistency coefficient values.

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