TY - JOUR
T1 - Evaluation of kidney injury biomarkers in rat amniotic fluid after gestational exposure to cadmium
AU - Jacobo-Estrada, Tania
AU - Cardenas-Gonzalez, Mariana
AU - Santoyo-Sánchez, Mitzi
AU - Parada-Cruz, Benjamín
AU - Uria-Galicia, Esther
AU - Arreola-Mendoza, Laura
AU - Barbier, Olivier
N1 - Publisher Copyright:
Copyright © 2016 John Wiley & Sons, Ltd.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Cadmium is a well-characterized nephrotoxic agent that is also capable of accumulating and diffusing across the placenta; however, only a few studies have addressed its effects over fetal kidneys and none of them has used a panel of sensitive and specific biomarkers for the detection of kidney injury. The goal of this study was to determine cadmium renal effects in rat fetuses by the quantification of early kidney injury biomarkers. Pregnant Wistar rats were exposed by inhalation to an isotonic saline solution or to CdCl2 solution (DDel=1.48 mg Cd kg−1 day−1) during gestational days (GD) 8–20. On GD 21, dams were euthanized and samples obtained. Kidney injury biomarkers were quantified in amniotic fluid samples and fetal kidneys were microscopically evaluated to search for histological alterations. Our results showed that cadmium exposure significantly raised albumin, osteopontin, vascular endothelial growth factor and tissue inhibitor of metalloproteinases-1 levels in amniotic fluid, whereas it decreased creatinine. Clusterin, calbindin and IFN-inducible protein 10 did not show any change. Accordingly, histological findings showed tubular damage and precipitations in the renal pelvis. In conclusion, gestational exposure to cadmium induces structural alterations in fetal renal tissue that can be detected by some kidney injury biomarkers in amniotic fluid samples.
AB - Cadmium is a well-characterized nephrotoxic agent that is also capable of accumulating and diffusing across the placenta; however, only a few studies have addressed its effects over fetal kidneys and none of them has used a panel of sensitive and specific biomarkers for the detection of kidney injury. The goal of this study was to determine cadmium renal effects in rat fetuses by the quantification of early kidney injury biomarkers. Pregnant Wistar rats were exposed by inhalation to an isotonic saline solution or to CdCl2 solution (DDel=1.48 mg Cd kg−1 day−1) during gestational days (GD) 8–20. On GD 21, dams were euthanized and samples obtained. Kidney injury biomarkers were quantified in amniotic fluid samples and fetal kidneys were microscopically evaluated to search for histological alterations. Our results showed that cadmium exposure significantly raised albumin, osteopontin, vascular endothelial growth factor and tissue inhibitor of metalloproteinases-1 levels in amniotic fluid, whereas it decreased creatinine. Clusterin, calbindin and IFN-inducible protein 10 did not show any change. Accordingly, histological findings showed tubular damage and precipitations in the renal pelvis. In conclusion, gestational exposure to cadmium induces structural alterations in fetal renal tissue that can be detected by some kidney injury biomarkers in amniotic fluid samples.
KW - TIMP-1
KW - VEGF
KW - fetal nephrotoxicity
KW - in utero exposure
KW - metals
KW - osteopontin
UR - http://www.scopus.com/inward/record.url?scp=84978805762&partnerID=8YFLogxK
U2 - 10.1002/jat.3286
DO - 10.1002/jat.3286
M3 - Artículo
SN - 0260-437X
VL - 36
SP - 1183
EP - 1193
JO - Journal of Applied Toxicology
JF - Journal of Applied Toxicology
IS - 9
ER -