Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model

Mirza Imran Shahzad, Naila Shahid, Nuzhat Sial, Abul Hasanat, Madiha Khalid, Maryam Yousaf, Zulfiqar Hyder, Muhammad Ashfaq, Gildardo Rivera, Azra Khanum

Research output: Contribution to journalArticle

Abstract

Tuberculosis (TB) is a life threatening infectious disease which is prevalent throughout the world. Mycobacterium bovis based Bacille Calmette-Gue´rin (BCG) is the only vaccine available against TB however, this (single) vaccine is not enough to eradicate it. Furthermore, numbers of researches from different parts of the World have shown its efficacy as variable. Hence other (better) vaccines like DNA vaccines are needed in addition to BCG in order to achieve desired goal of TB eradication. The current study was aimed to develop subunit based DNA vaccines against TB and to check their efficacy. Two constructs Bfrb-pND14 and Mpt32-pND14 were made and used as DNA vaccines. Endotoxin free DNA preparations were made and used in immunization studies. Twenty Balb/c female mice of age eight weeks were used in trial. Two experimental groups each comprising eight animals were used to inoculate Mpt32-pND14 and Bfrb-pND14 vaccines respectively. A group of four animals was used as negative control. Animals were bled through tail periodically and finally through cardiac puncture before euthanization. Antibodies were confirmed through dot blot and Agar Gel Immuno Diffusion test (AGID). All the animals immunized with both vaccines were found positive as tested through dot blot and AGID. The results of this study have indicated that both the M. tb genes have produced strong immune response in mice model through pND14 vector and proved themselves as good subunit based DNA vaccines.

Original languageEnglish
Pages (from-to)2025-2029
Number of pages5
JournalPakistan journal of pharmaceutical sciences
Volume30
Issue number5
StatePublished - 1 Sep 2017

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DNA Vaccines
Vaccines
Tuberculosis
Genes
Immunodiffusion
Agar
Mycobacterium bovis
Punctures
Endotoxins
Communicable Diseases
Tail
Immunization
Antibodies
DNA
Research

Cite this

Shahzad, M. I., Shahid, N., Sial, N., Hasanat, A., Khalid, M., Yousaf, M., ... Khanum, A. (2017). Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model. Pakistan journal of pharmaceutical sciences, 30(5), 2025-2029.
Shahzad, Mirza Imran ; Shahid, Naila ; Sial, Nuzhat ; Hasanat, Abul ; Khalid, Madiha ; Yousaf, Maryam ; Hyder, Zulfiqar ; Ashfaq, Muhammad ; Rivera, Gildardo ; Khanum, Azra. / Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model. In: Pakistan journal of pharmaceutical sciences. 2017 ; Vol. 30, No. 5. pp. 2025-2029.
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Shahzad, MI, Shahid, N, Sial, N, Hasanat, A, Khalid, M, Yousaf, M, Hyder, Z, Ashfaq, M, Rivera, G & Khanum, A 2017, 'Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model', Pakistan journal of pharmaceutical sciences, vol. 30, no. 5, pp. 2025-2029.

Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model. / Shahzad, Mirza Imran; Shahid, Naila; Sial, Nuzhat; Hasanat, Abul; Khalid, Madiha; Yousaf, Maryam; Hyder, Zulfiqar; Ashfaq, Muhammad; Rivera, Gildardo; Khanum, Azra.

In: Pakistan journal of pharmaceutical sciences, Vol. 30, No. 5, 01.09.2017, p. 2025-2029.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model

AU - Shahzad, Mirza Imran

AU - Shahid, Naila

AU - Sial, Nuzhat

AU - Hasanat, Abul

AU - Khalid, Madiha

AU - Yousaf, Maryam

AU - Hyder, Zulfiqar

AU - Ashfaq, Muhammad

AU - Rivera, Gildardo

AU - Khanum, Azra

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AB - Tuberculosis (TB) is a life threatening infectious disease which is prevalent throughout the world. Mycobacterium bovis based Bacille Calmette-Gue´rin (BCG) is the only vaccine available against TB however, this (single) vaccine is not enough to eradicate it. Furthermore, numbers of researches from different parts of the World have shown its efficacy as variable. Hence other (better) vaccines like DNA vaccines are needed in addition to BCG in order to achieve desired goal of TB eradication. The current study was aimed to develop subunit based DNA vaccines against TB and to check their efficacy. Two constructs Bfrb-pND14 and Mpt32-pND14 were made and used as DNA vaccines. Endotoxin free DNA preparations were made and used in immunization studies. Twenty Balb/c female mice of age eight weeks were used in trial. Two experimental groups each comprising eight animals were used to inoculate Mpt32-pND14 and Bfrb-pND14 vaccines respectively. A group of four animals was used as negative control. Animals were bled through tail periodically and finally through cardiac puncture before euthanization. Antibodies were confirmed through dot blot and Agar Gel Immuno Diffusion test (AGID). All the animals immunized with both vaccines were found positive as tested through dot blot and AGID. The results of this study have indicated that both the M. tb genes have produced strong immune response in mice model through pND14 vector and proved themselves as good subunit based DNA vaccines.

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M3 - Artículo

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VL - 30

SP - 2025

EP - 2029

JO - Pakistan journal of pharmaceutical sciences

JF - Pakistan journal of pharmaceutical sciences

SN - 1011-601X

IS - 5

ER -

Shahzad MI, Shahid N, Sial N, Hasanat A, Khalid M, Yousaf M et al. Evaluation of DNA vaccines encoding M. Tb gene Bfrb and Mpt32 in mice model. Pakistan journal of pharmaceutical sciences. 2017 Sep 1;30(5):2025-2029.