TY - JOUR
T1 - Ethanol tolerance is decreased by fructose in Saccharomyces and non-Saccharomyces yeasts
AU - De la Torre-González, Francisco Javier
AU - Narváez-Zapata, José Alberto
AU - López-y-López, Víctor Eric
AU - Larralde-Corona, Claudia Patricia
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - The maximal values of osmotic (fructose) stress and ethanol tolerances dependence on hexose type present in media were quantified for a collection of yeasts isolated from mezcal covering ten different genera, including Saccharomyces. The yeasts clustered in five groups where in the least tolerant group yeasts were not able to grow at a fructose concentration above 200 g/l, as compared to yeasts in the most tolerant group that were able to grew at concentration of fructose above 700 g/l. In ethanol agar plates without a carbon source, the maximum tolerance was of 9% v/v of ethanol for all of the yeasts. When ethanol was combined with glucose (20 g/l), a number of Saccharomyces cerevisiae strains were able to grow at up to 15% v/v ethanol, whereas the maximum was 10% v/v ethanol for the non-Saccharomyces yeasts. However, when fructose was used instead of glucose, none of the yeasts tested was able to grow on plates containing above 9% v/v ethanol, including S. cerevisiae. Hence fructose did not improve the tolerance to ethanol as observed for glucose, but rather fructose acted as an inhibitor or increasing the toxicity of ethanol.
AB - The maximal values of osmotic (fructose) stress and ethanol tolerances dependence on hexose type present in media were quantified for a collection of yeasts isolated from mezcal covering ten different genera, including Saccharomyces. The yeasts clustered in five groups where in the least tolerant group yeasts were not able to grow at a fructose concentration above 200 g/l, as compared to yeasts in the most tolerant group that were able to grew at concentration of fructose above 700 g/l. In ethanol agar plates without a carbon source, the maximum tolerance was of 9% v/v of ethanol for all of the yeasts. When ethanol was combined with glucose (20 g/l), a number of Saccharomyces cerevisiae strains were able to grow at up to 15% v/v ethanol, whereas the maximum was 10% v/v ethanol for the non-Saccharomyces yeasts. However, when fructose was used instead of glucose, none of the yeasts tested was able to grow on plates containing above 9% v/v ethanol, including S. cerevisiae. Hence fructose did not improve the tolerance to ethanol as observed for glucose, but rather fructose acted as an inhibitor or increasing the toxicity of ethanol.
KW - Drop-plate technique
KW - Ethanol tolerance
KW - Fructose
KW - Mezcal yeasts
UR - http://www.scopus.com/inward/record.url?scp=84953396246&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2015.11.024
DO - 10.1016/j.lwt.2015.11.024
M3 - Artículo
SN - 0023-6438
VL - 67
SP - 1
EP - 7
JO - LWT
JF - LWT
ER -