TY - JOUR
T1 - Establishment of in vitro germination of Distichlis spicata and response to osmotic stress
AU - Castelán-Fentanes, María Luisa
AU - García-Morales, Soledad
AU - Contreras-Jiménez, José Luis
AU - Ríos-Meléndez, Selma
AU - Bibbins-Martínez, Martha Dolores
AU - Villalobos-López, Miguel Angel
AU - Arroyo-Becerra, Analilia
N1 - Publisher Copyright:
© 2023 María Luisa Castelán-Fentanes et al.
PY - 2023
Y1 - 2023
N2 - Distichlis spicata var. stricta (L) Greene is a grass able to grow during drought or flooding seasons, as well as in poor, saline, and alkaline soils, making it an ideal model system to study salinity and water stress tolerance in plants. However, it is known to have an extremely low germination rate in the field, as low as 3%. Here, we established in vitro conditions to study the factors that affect D. spicata under osmotic and salt stress. Treatments that included mechanical scarification, 4°C stratification, and 20% NaDCC (w/v) disinfection significantly increased the germination rate up to 84% in only 5 days. Furthermore, saltgrass had good seed germination under high salinity (ionic) stress caused by 150 and 200 mM NaCl (50% and 30%, respectively). Saltgrass was sensitive to osmotic agents Mannitol and Sorbitol during the germination stage, suggesting that this is a main limiting developmental stage for D. spicata success under osmotic environments.
AB - Distichlis spicata var. stricta (L) Greene is a grass able to grow during drought or flooding seasons, as well as in poor, saline, and alkaline soils, making it an ideal model system to study salinity and water stress tolerance in plants. However, it is known to have an extremely low germination rate in the field, as low as 3%. Here, we established in vitro conditions to study the factors that affect D. spicata under osmotic and salt stress. Treatments that included mechanical scarification, 4°C stratification, and 20% NaDCC (w/v) disinfection significantly increased the germination rate up to 84% in only 5 days. Furthermore, saltgrass had good seed germination under high salinity (ionic) stress caused by 150 and 200 mM NaCl (50% and 30%, respectively). Saltgrass was sensitive to osmotic agents Mannitol and Sorbitol during the germination stage, suggesting that this is a main limiting developmental stage for D. spicata success under osmotic environments.
KW - Saltgrass
KW - in vitro germination
KW - osmotic stress
KW - salt stress
UR - http://www.scopus.com/inward/record.url?scp=85150500529&partnerID=8YFLogxK
U2 - 10.1163/22238980-bja10075
DO - 10.1163/22238980-bja10075
M3 - Artículo
AN - SCOPUS:85150500529
SN - 0792-9978
VL - 69
SP - 1
EP - 9
JO - Israel Journal of Plant Sciences
JF - Israel Journal of Plant Sciences
IS - 3
ER -