TY - JOUR
T1 - Enzymatic formulation strategies unlock highly-efficient saccharification of distinct pretreated corncobs
AU - Zhang, Yu
AU - Wang, Ruonan
AU - Yang, Jinshui
AU - Liu, Liang
AU - Yang, Yi
AU - Wang, Entao
AU - Yuan, Hongli
N1 - Publisher Copyright:
© 2022
PY - 2022/11/1
Y1 - 2022/11/1
N2 - Optimization of the composition of enzyme cocktail is an effective way to improve the hydrolytic efficiency and reduce the cost during enzymatic hydrolysis of lignocellulose. Herein, the optimal enzymes ratios of enzyme cocktails for efficient hydrolysis of corncobs with distinct pretreatments were quantitatively formulated. As the importance of pretreatment type on xylan extraction, the optimized cellulase/hemicellulase ratios for NaClO2, H2SO4 and steam explosion treated corncob were 1:1.5, 1:0.15 and 1:0.75, respectively. Meanwhile, different requirements for types of cellulases are also determined: endoglucanase and cellobiohydrolase were key enzymes in the hydrolysis of NaClO2 and H2SO4 treated corncobs, respectively, while the synergy of three kinds of cellulases was preferred for steam-exploded corncob. Furthermore, based on the optimized cellulase/hemicellulase ratios, cellulases proportions for hydrolyzing the corncobs pretreated with distinctive methods were determined. Notably, by the enzymatic formulation strategy, 100% yields of glucose and xylose were obtained simultaneously from NaClO2 treated corncob, which was up to 70.1% and 80.1% at high solid loading (20%), respectively. Without detoxification of hydrolysate, high isopropanol yield of 0.45 g/g (of dry matter) was achieved. Hence, this study demonstrated a low cost and high efficient conversion strategy for prospective high-value utilization of lignocellulose.
AB - Optimization of the composition of enzyme cocktail is an effective way to improve the hydrolytic efficiency and reduce the cost during enzymatic hydrolysis of lignocellulose. Herein, the optimal enzymes ratios of enzyme cocktails for efficient hydrolysis of corncobs with distinct pretreatments were quantitatively formulated. As the importance of pretreatment type on xylan extraction, the optimized cellulase/hemicellulase ratios for NaClO2, H2SO4 and steam explosion treated corncob were 1:1.5, 1:0.15 and 1:0.75, respectively. Meanwhile, different requirements for types of cellulases are also determined: endoglucanase and cellobiohydrolase were key enzymes in the hydrolysis of NaClO2 and H2SO4 treated corncobs, respectively, while the synergy of three kinds of cellulases was preferred for steam-exploded corncob. Furthermore, based on the optimized cellulase/hemicellulase ratios, cellulases proportions for hydrolyzing the corncobs pretreated with distinctive methods were determined. Notably, by the enzymatic formulation strategy, 100% yields of glucose and xylose were obtained simultaneously from NaClO2 treated corncob, which was up to 70.1% and 80.1% at high solid loading (20%), respectively. Without detoxification of hydrolysate, high isopropanol yield of 0.45 g/g (of dry matter) was achieved. Hence, this study demonstrated a low cost and high efficient conversion strategy for prospective high-value utilization of lignocellulose.
KW - Corncob
KW - Enzyme formulation
KW - Isopropanol
KW - Lignocellulosic biomass
KW - Pretreatment
KW - Saccharification
UR - http://www.scopus.com/inward/record.url?scp=85133855549&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2022.115320
DO - 10.1016/j.indcrop.2022.115320
M3 - Artículo
AN - SCOPUS:85133855549
SN - 0926-6690
VL - 187
JO - Industrial Crops and Products
JF - Industrial Crops and Products
M1 - 115320
ER -