Entamoeba histolytica: Proteinase secretion induced by collagen type I is dependent on cytoskeleton integrity

José De Jesús Serrano, Mireya De La Garza, Magda Reyes, Gloria León, Rosalinda Tovar, María De Lourdes Muñoz

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19 Citations (Scopus)

Abstract

Proteolytic activities of the protozoan parasite Entamoeba histolytica strain HM1:IMSS and the cytochalasin D-resistant mutant BG-3 were analyzed following stimulation with collagen type I and Ca2+, which induces electron-dense associated collagenase secretion. The mutant BG-3 had a protease activity of 73 kDa and secretion of total protease activity was not stimulated by collagen type I and Ca2+, which produced, in contrast, a 2-fold increase in protease secretion by the parental strain. This collagen-stimulated protease secretion was inhibited by cytochalasin D at a concentration of 1 μg/ml. Cytochalasin D did not have any effect on the protease activity released by the mutant BG-3. These findings suggest that cytoskeleton integrity is necessary for collagen-induced protease secretion.
Original languageAmerican English
Pages (from-to)200-205
Number of pages179
JournalParasitology Research
DOIs
StatePublished - 13 Mar 1996
Externally publishedYes

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Entamoeba histolytica
protease
secretions
collagens
Collagen Type I
cytoskeleton
Cytoskeleton
Collagen
integrity
collagen
Peptide Hydrolases
proteinases
secretion
cytochalasin D
Cytochalasin D
mutants
calcium
parasites
collagenase
Collagenases

Cite this

De Jesús Serrano, José ; De La Garza, Mireya ; Reyes, Magda ; León, Gloria ; Tovar, Rosalinda ; De Lourdes Muñoz, María. / Entamoeba histolytica: Proteinase secretion induced by collagen type I is dependent on cytoskeleton integrity. In: Parasitology Research. 1996 ; pp. 200-205.
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abstract = "Proteolytic activities of the protozoan parasite Entamoeba histolytica strain HM1:IMSS and the cytochalasin D-resistant mutant BG-3 were analyzed following stimulation with collagen type I and Ca2+, which induces electron-dense associated collagenase secretion. The mutant BG-3 had a protease activity of 73 kDa and secretion of total protease activity was not stimulated by collagen type I and Ca2+, which produced, in contrast, a 2-fold increase in protease secretion by the parental strain. This collagen-stimulated protease secretion was inhibited by cytochalasin D at a concentration of 1 μg/ml. Cytochalasin D did not have any effect on the protease activity released by the mutant BG-3. These findings suggest that cytoskeleton integrity is necessary for collagen-induced protease secretion.",
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Entamoeba histolytica: Proteinase secretion induced by collagen type I is dependent on cytoskeleton integrity. / De Jesús Serrano, José; De La Garza, Mireya; Reyes, Magda; León, Gloria; Tovar, Rosalinda; De Lourdes Muñoz, María.

In: Parasitology Research, 13.03.1996, p. 200-205.

Research output: Contribution to journalArticle

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AU - De Jesús Serrano, José

AU - De La Garza, Mireya

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AU - Tovar, Rosalinda

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