Encapsulation efficiency of CdSe/ZnS quantum dots by liposomes determined by thermal lens microscopy

Jessica Batalla; Humberto Cabrera; Eduardo San Martín-Martínez; Dorota Korte; Antonio Calderón; Ernesto Marín

doi:10.1364/BOE.6.003898

Encapsulation efficiency of CdSe/ZnS quantum dots by liposomes determined by thermal lens microscopy

Jessica Batalla, Humberto Cabrera, Eduardo San Martín-Martínez, Dorota Korte, Antonio Calderón, Ernesto Marín

Research output: Contribution to journal › Article › peer-review

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Abstract

In this study the encapsulation of core shell carboxyl CdSe/ZnS quantum dots (QDs) by phospholipids liposome complexes is presented. It makes the quantum dots water soluble and photo-stable. Fluorescence selfquenching of the QDs inside the liposomes was observed. Therefore, the thermal lens microscopy (TLM) was found to be an useful tool for measuring the encapsulation efficiency of the QDs by the liposomes, for which an optimum value of 36% was determined. The obtained limit of detection (LOD) for determining QDs concentration by TLM was 0.13 nM. Moreover, the encapsulated QDs showed no prominent cytotoxicity toward Breast cancer cells line MDA-MB-231. This study was supported by UVvisible spectroscopy, high resolution transmission electron microscopy (HRTEM) and dynamic light scattering measurements (DLS).

Original language	English
Article number	A016
Pages (from-to)	3898-3906
Number of pages	9
Journal	Biomedical Optics Express
Volume	6
Issue number	10
DOIs	https://doi.org/10.1364/BOE.6.003898
State	Published - 1 Oct 2015
Externally published	Yes

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title = "Encapsulation efficiency of CdSe/ZnS quantum dots by liposomes determined by thermal lens microscopy",

abstract = "In this study the encapsulation of core shell carboxyl CdSe/ZnS quantum dots (QDs) by phospholipids liposome complexes is presented. It makes the quantum dots water soluble and photo-stable. Fluorescence selfquenching of the QDs inside the liposomes was observed. Therefore, the thermal lens microscopy (TLM) was found to be an useful tool for measuring the encapsulation efficiency of the QDs by the liposomes, for which an optimum value of 36% was determined. The obtained limit of detection (LOD) for determining QDs concentration by TLM was 0.13 nM. Moreover, the encapsulated QDs showed no prominent cytotoxicity toward Breast cancer cells line MDA-MB-231. This study was supported by UVvisible spectroscopy, high resolution transmission electron microscopy (HRTEM) and dynamic light scattering measurements (DLS).",

author = "Jessica Batalla and Humberto Cabrera and Mart{\'i}n-Mart{\'i}nez, {Eduardo San} and Dorota Korte and Antonio Calder{\'o}n and Ernesto Mar{\'i}n",

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T1 - Encapsulation efficiency of CdSe/ZnS quantum dots by liposomes determined by thermal lens microscopy

AU - Batalla, Jessica

AU - Cabrera, Humberto

AU - Martín-Martínez, Eduardo San

AU - Korte, Dorota

AU - Calderón, Antonio

AU - Marín, Ernesto

PY - 2015/10/1

Y1 - 2015/10/1

N2 - In this study the encapsulation of core shell carboxyl CdSe/ZnS quantum dots (QDs) by phospholipids liposome complexes is presented. It makes the quantum dots water soluble and photo-stable. Fluorescence selfquenching of the QDs inside the liposomes was observed. Therefore, the thermal lens microscopy (TLM) was found to be an useful tool for measuring the encapsulation efficiency of the QDs by the liposomes, for which an optimum value of 36% was determined. The obtained limit of detection (LOD) for determining QDs concentration by TLM was 0.13 nM. Moreover, the encapsulated QDs showed no prominent cytotoxicity toward Breast cancer cells line MDA-MB-231. This study was supported by UVvisible spectroscopy, high resolution transmission electron microscopy (HRTEM) and dynamic light scattering measurements (DLS).

AB - In this study the encapsulation of core shell carboxyl CdSe/ZnS quantum dots (QDs) by phospholipids liposome complexes is presented. It makes the quantum dots water soluble and photo-stable. Fluorescence selfquenching of the QDs inside the liposomes was observed. Therefore, the thermal lens microscopy (TLM) was found to be an useful tool for measuring the encapsulation efficiency of the QDs by the liposomes, for which an optimum value of 36% was determined. The obtained limit of detection (LOD) for determining QDs concentration by TLM was 0.13 nM. Moreover, the encapsulated QDs showed no prominent cytotoxicity toward Breast cancer cells line MDA-MB-231. This study was supported by UVvisible spectroscopy, high resolution transmission electron microscopy (HRTEM) and dynamic light scattering measurements (DLS).

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DO - 10.1364/BOE.6.003898

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

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ER -

Encapsulation efficiency of CdSe/ZnS quantum dots by liposomes determined by thermal lens microscopy

Abstract

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