TY - JOUR
T1 - Electronic effects in emission of core/shell CdSe/ZnS quantum dots conjugated to anti-Interleukin 10 antibodies
AU - Vazquez, A. L.Quintos
AU - Torchynska, T. V.
AU - Casas Espinola, J. L.
AU - Gómez, J. A.Jaramillo
AU - Douda, J.
N1 - Funding Information:
The work was partially supported by CONACYT Mexico (Project 130387 ) and by SIP-IPN, Mexico .
PY - 2013
Y1 - 2013
N2 - The paper presents a comparative study of the photoluminescence (PL) and Raman scattering spectra of the core-shell CdSe/ZnS quantum dots (QDs) in nonconjugated states and after the conjugation to anti-Interleukin 10 antibodies (anti-IL10). All optical measurements are performed on the dried droplets of the original solution of nonconjugated and bioconjugated QDs located on the Si substrate. CdSe/ZnS QDs with emission at 605 and 655 nm have been used. PL spectra of nonconjugated QDs are characterized by one Gaussian shape PL band related to the exciton emission in the CdSe core. PL spectra of bioconjugated QDs have changed essentially: the core PL band shifts into the high energy spectral range ("blue" sift) and becomes asymmetric. Additionally two new PL bands appear. A set of physical reasons has been proposed for the "blue" shift explanation for the core PL band in bioconjugated QDs. Then Raman scattering spectra have been studied with the aim to analyze the impact of elastic strains or the oxidation process at the QD bioconjugation. The variation of PL spectra versus excitation light intensities has been studied to analyze the exciton emission via excited states in QDs. Finally the PL spectrum transformation for the core emission in bioconjugated QDs has been attributed to the electronic quantum confined effects stimulated by the electric charges of bioconjugated antibodies.
AB - The paper presents a comparative study of the photoluminescence (PL) and Raman scattering spectra of the core-shell CdSe/ZnS quantum dots (QDs) in nonconjugated states and after the conjugation to anti-Interleukin 10 antibodies (anti-IL10). All optical measurements are performed on the dried droplets of the original solution of nonconjugated and bioconjugated QDs located on the Si substrate. CdSe/ZnS QDs with emission at 605 and 655 nm have been used. PL spectra of nonconjugated QDs are characterized by one Gaussian shape PL band related to the exciton emission in the CdSe core. PL spectra of bioconjugated QDs have changed essentially: the core PL band shifts into the high energy spectral range ("blue" sift) and becomes asymmetric. Additionally two new PL bands appear. A set of physical reasons has been proposed for the "blue" shift explanation for the core PL band in bioconjugated QDs. Then Raman scattering spectra have been studied with the aim to analyze the impact of elastic strains or the oxidation process at the QD bioconjugation. The variation of PL spectra versus excitation light intensities has been studied to analyze the exciton emission via excited states in QDs. Finally the PL spectrum transformation for the core emission in bioconjugated QDs has been attributed to the electronic quantum confined effects stimulated by the electric charges of bioconjugated antibodies.
KW - Anti-Interleukin 10 antibodies
KW - Bioconjugation
KW - CdSe/ZnS quantum dots
KW - QD emission and Raman scattering
UR - http://www.scopus.com/inward/record.url?scp=84877866208&partnerID=8YFLogxK
U2 - 10.1016/j.jlumin.2013.04.022
DO - 10.1016/j.jlumin.2013.04.022
M3 - Artículo
SN - 0022-2313
VL - 143
SP - 38
EP - 42
JO - Journal of Luminescence
JF - Journal of Luminescence
ER -