Electronic effects in CdSe/ZnS quantum dots conjugated to IL-10 antibodies

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Abstract

The paper presents the study of photoluminescence (PL) and Raman scattering spectra of nonconjugated and bioconjugated CdSe/ZnS core-shell quantum dots (QDs). CdSe/ZnS QDs used are characterized by color emission with the maxima at 605 nm (2.05eV) and 655 nm (1.89 eV). The QD conjugation has been performed with biomolecules - the anti Interleukin 10 antibodies (anti IL10 mAb). PL spectra of nonconjugated QDs are characterized by one symmetric PL band related to exciton emission in the CdSe core of QDs. PL spectra of bioconjugated QDs have changed essentially: PL band shifts into high energy side and becomes asymmetric. To explain last effect the model has been proposed which assumes that the PL spectrum transformation in bioconjugated QDs is connected with the Stark effect. To confirm the existence of electric dipoles in anti IL-10 mAb the Raman scattering spectra have been investigated. The enhancement of Raman scattering is observed in bioconjugated CdSe/ZnS QDs. The last effect testifies that the IL10 antibodies are characterized, actually, by the electric dipole moment that permits them to interact with an electric field of excitation light and to provoke the surface enhanced Raman scattering (SERS) and the Stark effects in QDs.

Translated title of the contributionEfectos electrónicos en puntos cuánticos CdSe/ZnS conjugados con anticuerpos IL-10
Original languageEnglish
Title of host publicationTechnical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
Pages90-93
Number of pages4
StatePublished - 2012
EventNanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012 - Santa Clara, CA, United States
Duration: 18 Jun 201221 Jun 2012

Publication series

NameTechnical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012

Conference

ConferenceNanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
Country/TerritoryUnited States
CitySanta Clara, CA
Period18/06/1221/06/12

Keywords

  • Anti interleukin 10 antibody
  • Bioconjugation
  • CdSe/ZnS quantum dots
  • Photoluminescence
  • Raman scattering

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