TY - JOUR
T1 - Effects on Cell Growth, Lipid and Biochemical Composition of Thalassiosira weissflogii (Bacillariophyceae) Cultured under Two Nitrogen Sources
AU - Hernández-Sandoval, Francisco Eduardo
AU - Del Ángel-Rodríguez, Jorge Arturo
AU - Núñez-Vázquez, Erick Julian
AU - Band-Schmidt, Christine Johanna
AU - Arredondo-Vega, Bertha Olivia
AU - Campa-Córdova, Ángel Isidro
AU - Moreno-Legorreta, Manuel
AU - Fernández-Herrera, Leyberth José
AU - López-Cortés, David Javier
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - The protein and polyunsaturated fatty acid (PUFA) enrichment of microalgae can improve their nutritional value for larvae of various reared organisms. Protein enrichment can be achieved by increasing nitrogen concentration and selecting nitrogen sources that are easy to assimilate during microalga culture. Nitrogen-rich cultures can increase organism growth, biomass, and protein content, but their lipid content tends to stall. Since the diatom Thalassiosira weissflogii is usually provided to feed shrimp larvae, this study evaluated its digestibility and biochemical composition, culturing it with two nitrogen sources (NaNO3 and NH4Cl) at different concentrations (111.25, 222.50, 445 and 890 µM). The cell abundance, dry-weight biomass, Chl a, proteins, carbohydrates, total lipids and essential fatty acids were determined. The cell density and biomass peaked faster (day 12) with treatment < 890 µM than with 890 µM (day 15) in both nitrogen sources. However, the highest cell density, biomass and peak protein yield were not significantly different among treatments, suggesting the need to compare maintenance costs for a given production. After nine days of culture, concentrations ≤ 222.5 µM increased lipid content irrespective of the nitrogen source and decreased by 10–20% afterwards. With higher lipid production, the dominant PUFA were eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). One gram of NH4Cl provides ~60% more nitrogen than 1 g of NaNO3 . In conclusion, based on time and growth rate, T. weissflogii cultivated with NH4Cl at 222.50 µM produced EPA and DHA at a better yield–cost ratio for biomass and lipid production. Furthermore, its nutritional value as enriched live-food for the reared larvae of marine organisms suggests potential biotechnological applications for aquaculture.
AB - The protein and polyunsaturated fatty acid (PUFA) enrichment of microalgae can improve their nutritional value for larvae of various reared organisms. Protein enrichment can be achieved by increasing nitrogen concentration and selecting nitrogen sources that are easy to assimilate during microalga culture. Nitrogen-rich cultures can increase organism growth, biomass, and protein content, but their lipid content tends to stall. Since the diatom Thalassiosira weissflogii is usually provided to feed shrimp larvae, this study evaluated its digestibility and biochemical composition, culturing it with two nitrogen sources (NaNO3 and NH4Cl) at different concentrations (111.25, 222.50, 445 and 890 µM). The cell abundance, dry-weight biomass, Chl a, proteins, carbohydrates, total lipids and essential fatty acids were determined. The cell density and biomass peaked faster (day 12) with treatment < 890 µM than with 890 µM (day 15) in both nitrogen sources. However, the highest cell density, biomass and peak protein yield were not significantly different among treatments, suggesting the need to compare maintenance costs for a given production. After nine days of culture, concentrations ≤ 222.5 µM increased lipid content irrespective of the nitrogen source and decreased by 10–20% afterwards. With higher lipid production, the dominant PUFA were eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). One gram of NH4Cl provides ~60% more nitrogen than 1 g of NaNO3 . In conclusion, based on time and growth rate, T. weissflogii cultivated with NH4Cl at 222.50 µM produced EPA and DHA at a better yield–cost ratio for biomass and lipid production. Furthermore, its nutritional value as enriched live-food for the reared larvae of marine organisms suggests potential biotechnological applications for aquaculture.
KW - Algal application
KW - Biochemical composition
KW - Biotechnological aquaculture
KW - Fatty acid
KW - Nitrogen source
UR - http://www.scopus.com/inward/record.url?scp=85122995920&partnerID=8YFLogxK
U2 - 10.3390/app12030961
DO - 10.3390/app12030961
M3 - Artículo
AN - SCOPUS:85122995920
SN - 2076-3417
VL - 12
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
IS - 3
M1 - 961
ER -