TY - JOUR
T1 - Effects of a Natural Extract of Aronia melanocarpa Berry on Endothelial Cell Nitric Oxide Production
AU - Varela, Claudia Elena
AU - Fromentin, Emilie
AU - Roller, Marc
AU - Villarreal, Francisco
AU - Ramirez-Sanchez, Israel
N1 - Publisher Copyright:
© 2015 Wiley Periodicals, Inc.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The effects of acute and chronic treatment with Aronia extracts on nitric oxide (NO) production and endothelial nitric oxide synthase (eNOS) phosphorylation in bovine coronary artery endothelial cells were investigated. Acute time course and concentration–response experiments were performed to determine the time and concentration at which Aronia induced maximal NO synthesis and eNOS phosphorylation. The findings indicate that relatively low concentrations (0.1 μg/mL) of Aronia extract significantly induced NO synthesis and eNOS phosphorylation after 10 min of treatment. Increased sensitivity of eNOS and a significant increase in NO synthesis resulted from longer term stimulation with Aronia (48 h) and an acute re-treatment of the cells (10 min). Practical Applications: These in vitro results may be translated into potential future clinical applications where Aronia extracts may be used for prevention and coadjuvant treatment of cardiovascular diseases via increases in endothelial nitric oxide synthesis and related improvements in vascular functions. Given the dose–response effect of Aronia extract in vitro and metabolism of polyphenols that occurs in humans, dose–response studies would be necessary to define the optimal daily amount to be consumed.
AB - The effects of acute and chronic treatment with Aronia extracts on nitric oxide (NO) production and endothelial nitric oxide synthase (eNOS) phosphorylation in bovine coronary artery endothelial cells were investigated. Acute time course and concentration–response experiments were performed to determine the time and concentration at which Aronia induced maximal NO synthesis and eNOS phosphorylation. The findings indicate that relatively low concentrations (0.1 μg/mL) of Aronia extract significantly induced NO synthesis and eNOS phosphorylation after 10 min of treatment. Increased sensitivity of eNOS and a significant increase in NO synthesis resulted from longer term stimulation with Aronia (48 h) and an acute re-treatment of the cells (10 min). Practical Applications: These in vitro results may be translated into potential future clinical applications where Aronia extracts may be used for prevention and coadjuvant treatment of cardiovascular diseases via increases in endothelial nitric oxide synthesis and related improvements in vascular functions. Given the dose–response effect of Aronia extract in vitro and metabolism of polyphenols that occurs in humans, dose–response studies would be necessary to define the optimal daily amount to be consumed.
UR - http://www.scopus.com/inward/record.url?scp=84981203315&partnerID=8YFLogxK
U2 - 10.1111/jfbc.12226
DO - 10.1111/jfbc.12226
M3 - Artículo
C2 - 27616799
SN - 0145-8884
VL - 40
SP - 404
EP - 410
JO - Journal of Food Biochemistry
JF - Journal of Food Biochemistry
IS - 4
ER -