TY - JOUR
T1 - Effect of transglutaminase cross-linking in protein isolates from a mixture of Two Quinoa varieties with chitosan on the physicochemical properties of edible films
AU - Escamilla-García, Monserrat
AU - Delgado-Sánchez, Luis Felipe
AU - Ríos-Romo, Raquel Adriana
AU - García-Almendárez, Blanca E.
AU - Calderón-Domínguez, Georgina
AU - VicenteMéndez-Méndez, Juan
AU - Amaro-Reyes, Aldo
AU - Di Pierro, Prospero
AU - Regalado-González, Carlos
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019
Y1 - 2019
N2 - The growing demand forminimally processed foodswith a long shelf life and environmentally friendly materials has forced industry to develop new technologies for food preservation and handling. The use of edible films has emerged as an alternative solution to this problem, and mixtures of carbohydrates and proteins, may be formulated to improve their properties. The objective of this work was to evaluate the effect of protein cross-linking with transglutaminase (TG) of two varieties of quinoa protein isolate (Chenopodium quinoa) [Willd (QW), and Pasankalla (QP)] on the physicochemical and barrier properties of edible films based on chitosan (CT)-quinoa protein. The evaluated properties were water vapor permeability (WVP), solubility, adsorption, roughness determined by atomic force microscopy, and the interactions among the main film components determined by Raman spectroscopy. The results indicated that TG interacted with lysine of QW and QP. CT:QW (1:5, w/w) showed the lowest solubility (14.02 ± 2.17% w/w). WVP varied with the composition of the mixture. The WVP of CT:quinoa protein ranged from 2.85 to 9.95 × 10-11 g cm Pa-1 cm-2 s-1 without TG, whereas adding TG reduced this range to 2.42-4.69 × 10-11 g cm Pa-1 cm-2 s-1. The addition of TG to CT:QP (1:10, w/w) reduced the film surface roughness from 8.0 ± 0.5 nm to 4.4 ± 0.3 nm. According to the sorption isotherm, the addition of TG to CT-QW films improved their stability [monolayer (Xm) = 0.13 ± 0.02 %]. Films with a higher amount of cross-linking showed the highest improvement in the evaluated physical properties, but interactions among proteins that were catalyzed by TG depended on the protein source and profile.
AB - The growing demand forminimally processed foodswith a long shelf life and environmentally friendly materials has forced industry to develop new technologies for food preservation and handling. The use of edible films has emerged as an alternative solution to this problem, and mixtures of carbohydrates and proteins, may be formulated to improve their properties. The objective of this work was to evaluate the effect of protein cross-linking with transglutaminase (TG) of two varieties of quinoa protein isolate (Chenopodium quinoa) [Willd (QW), and Pasankalla (QP)] on the physicochemical and barrier properties of edible films based on chitosan (CT)-quinoa protein. The evaluated properties were water vapor permeability (WVP), solubility, adsorption, roughness determined by atomic force microscopy, and the interactions among the main film components determined by Raman spectroscopy. The results indicated that TG interacted with lysine of QW and QP. CT:QW (1:5, w/w) showed the lowest solubility (14.02 ± 2.17% w/w). WVP varied with the composition of the mixture. The WVP of CT:quinoa protein ranged from 2.85 to 9.95 × 10-11 g cm Pa-1 cm-2 s-1 without TG, whereas adding TG reduced this range to 2.42-4.69 × 10-11 g cm Pa-1 cm-2 s-1. The addition of TG to CT:QP (1:10, w/w) reduced the film surface roughness from 8.0 ± 0.5 nm to 4.4 ± 0.3 nm. According to the sorption isotherm, the addition of TG to CT-QW films improved their stability [monolayer (Xm) = 0.13 ± 0.02 %]. Films with a higher amount of cross-linking showed the highest improvement in the evaluated physical properties, but interactions among proteins that were catalyzed by TG depended on the protein source and profile.
KW - Chitosan
KW - Edible films
KW - Quinoa protein
KW - Transglutaminase
UR - http://www.scopus.com/inward/record.url?scp=85075573497&partnerID=8YFLogxK
U2 - 10.3390/coatings9110736
DO - 10.3390/coatings9110736
M3 - Artículo
AN - SCOPUS:85075573497
SN - 2079-6412
VL - 9
JO - Coatings
JF - Coatings
IS - 11
M1 - 736
ER -