TY - JOUR
T1 - Efecto del tiempo de exposición a baja temperatura y de reguladores de crecimiento en la regeneración de plantas a partir de anteras de arroz oryza sativa l. (cultivar japónica h2005)
AU - Trejo-Tapia, Gabriela
AU - Maldonado-Amaya, Uriel
AU - Jiménez-Aparicio, Antonio
AU - Blanqueto-Illescas, Manuel
AU - Salcedo-Morales, Guadalupe
AU - Marínez-Bonfil, Bianca Patricia
AU - De Jesús-Sánchez, Antonia
PY - 2002
Y1 - 2002
N2 - The effect of exposition to 4°C (during 7,14 and 21 d) and different concentrations of several growth regulators on the regeneration of green plants, starting from anthers of rice (Oryza saliva L. Japónica H2005 cultivar) was studied. Results showed that treatment at 4°C for seven days stimulated the induction of callus starting from anthers. The plant growth regulators were essential for this induction, the largest effect was obtained with a combination of 2,4-D (1.5 mg L-1)and KIN (1 mg L-1); the highest formation of shoots and roots was achieved with the same auxin and BAP (0.5 mg L-1). The transfer of these materials to a culture media without 2,4-D allowed 37.5 % conversion to green haploids plants.
AB - The effect of exposition to 4°C (during 7,14 and 21 d) and different concentrations of several growth regulators on the regeneration of green plants, starting from anthers of rice (Oryza saliva L. Japónica H2005 cultivar) was studied. Results showed that treatment at 4°C for seven days stimulated the induction of callus starting from anthers. The plant growth regulators were essential for this induction, the largest effect was obtained with a combination of 2,4-D (1.5 mg L-1)and KIN (1 mg L-1); the highest formation of shoots and roots was achieved with the same auxin and BAP (0.5 mg L-1). The transfer of these materials to a culture media without 2,4-D allowed 37.5 % conversion to green haploids plants.
KW - Auxins
KW - Citokinins
KW - Cold treatment
KW - Haploids
KW - Oryza saliva L
UR - http://www.scopus.com/inward/record.url?scp=52449085758&partnerID=8YFLogxK
M3 - Artículo
SN - 1405-3195
VL - 36
SP - 441
EP - 449
JO - Agrociencia
JF - Agrociencia
IS - 4
ER -