TY - JOUR
T1 - Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica
AU - Velázquez-Domínguez, José
AU - A. Marchat, Laurence
AU - López-Camarillo, Cesar
AU - Mendoza-Hernández, Guillermo
AU - Sánchez-Espíndola, Esther
AU - Calzada, Fernando
AU - Ortega-Hernández, Alfredo
AU - Sánchez-Monroy, Virginia
AU - Ramírez-Moreno, Esther
N1 - Funding Information:
This work was supported by CONACyT , Secretaría de Investigación y Posgrado (SIP)-IPN , and Comisión de Operación y Fomento de Actividades Académicas (COFAA)-IPN , México. We thank Carlos Amir Carmona for his help in the quantification of ultrastructural changes, Dr. Mario Alberto Rodríguez and Dr. Patricia Talamás from CINVESTAV-IPN, México for the anti-E. histolytica PFO and anti-E. histolytica Lectin antibodies.
PY - 2013/11
Y1 - 2013/11
N2 - Entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. Although several drugs are effective against E. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. Medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. Incomptine A isolated from Decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in vitro activity against E. histolytica trophozoites. However the molecular mechanisms involved in its antiprotozoal activity are still unknown. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that 21 E. histolytica proteins were differentially expressed in response to incomptine A treatment. Notably, three glycolytic enzymes, namely enolase, pyruvate:ferredoxin oxidoreductase and fructose-1,6-biphosphate aldolase, were down-regulated. Moreover, ultrastructural analysis of trophozoites through electronic microscopy showed an increased number of glycogen granules. Taken together, our data suggested that incomptine A could affect E. histolytica growth through alteration of its energy metabolism.
AB - Entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. Although several drugs are effective against E. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. Medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. Incomptine A isolated from Decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in vitro activity against E. histolytica trophozoites. However the molecular mechanisms involved in its antiprotozoal activity are still unknown. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that 21 E. histolytica proteins were differentially expressed in response to incomptine A treatment. Notably, three glycolytic enzymes, namely enolase, pyruvate:ferredoxin oxidoreductase and fructose-1,6-biphosphate aldolase, were down-regulated. Moreover, ultrastructural analysis of trophozoites through electronic microscopy showed an increased number of glycogen granules. Taken together, our data suggested that incomptine A could affect E. histolytica growth through alteration of its energy metabolism.
KW - Amoebiasis
KW - Decachaeta incompta
KW - Glycolisis
KW - Proteomic analysis
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=84884359389&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2013.08.015
DO - 10.1016/j.exppara.2013.08.015
M3 - Artículo
C2 - 23994114
SN - 0014-4894
VL - 135
SP - 503
EP - 510
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -