Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica

José Velázquez-Domínguez, Laurence A. Marchat, Cesar López-Camarillo, Guillermo Mendoza-Hernández, Esther Sánchez-Espíndola, Fernando Calzada, Alfredo Ortega-Hernández, Virginia Sánchez-Monroy, Esther Ramírez-Moreno

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. Although several drugs are effective against E. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. Medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. Incomptine A isolated from Decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in vitro activity against E. histolytica trophozoites. However the molecular mechanisms involved in its antiprotozoal activity are still unknown. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that 21 E. histolytica proteins were differentially expressed in response to incomptine A treatment. Notably, three glycolytic enzymes, namely enolase, pyruvate:ferredoxin oxidoreductase and fructose-1,6-biphosphate aldolase, were down-regulated. Moreover, ultrastructural analysis of trophozoites through electronic microscopy showed an increased number of glycogen granules. Taken together, our data suggested that incomptine A could affect E. histolytica growth through alteration of its energy metabolism. © 2013 Elsevier Inc.
Original languageAmerican English
Pages (from-to)503-510
Number of pages451
JournalExperimental Parasitology
DOIs
StatePublished - 1 Nov 2013

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Entamoeba histolytica
Sesquiterpenes
Lactones
Energy Metabolism
Trophozoites
Amebiasis
Pyruvate Synthase
Fructose-Bisphosphate Aldolase
Electrospray Ionization Mass Spectrometry
Phosphopyruvate Hydratase
Electrophoresis, Gel, Two-Dimensional
Medicinal Plants
Complementary Therapies
Fructose
Glycogen
Proteomics
Developing Countries
Microscopy
incomptine A
Enzymes

Cite this

Velázquez-Domínguez, José ; A. Marchat, Laurence ; López-Camarillo, Cesar ; Mendoza-Hernández, Guillermo ; Sánchez-Espíndola, Esther ; Calzada, Fernando ; Ortega-Hernández, Alfredo ; Sánchez-Monroy, Virginia ; Ramírez-Moreno, Esther. / Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica. In: Experimental Parasitology. 2013 ; pp. 503-510.
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abstract = "Entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. Although several drugs are effective against E. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. Medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. Incomptine A isolated from Decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in vitro activity against E. histolytica trophozoites. However the molecular mechanisms involved in its antiprotozoal activity are still unknown. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that 21 E. histolytica proteins were differentially expressed in response to incomptine A treatment. Notably, three glycolytic enzymes, namely enolase, pyruvate:ferredoxin oxidoreductase and fructose-1,6-biphosphate aldolase, were down-regulated. Moreover, ultrastructural analysis of trophozoites through electronic microscopy showed an increased number of glycogen granules. Taken together, our data suggested that incomptine A could affect E. histolytica growth through alteration of its energy metabolism. {\circledC} 2013 Elsevier Inc.",
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Velázquez-Domínguez, J, A. Marchat, L, López-Camarillo, C, Mendoza-Hernández, G, Sánchez-Espíndola, E, Calzada, F, Ortega-Hernández, A, Sánchez-Monroy, V & Ramírez-Moreno, E 2013, 'Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica', Experimental Parasitology, pp. 503-510. https://doi.org/10.1016/j.exppara.2013.08.015

Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica. / Velázquez-Domínguez, José; A. Marchat, Laurence; López-Camarillo, Cesar; Mendoza-Hernández, Guillermo; Sánchez-Espíndola, Esther; Calzada, Fernando; Ortega-Hernández, Alfredo; Sánchez-Monroy, Virginia; Ramírez-Moreno, Esther.

In: Experimental Parasitology, 01.11.2013, p. 503-510.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica

AU - Velázquez-Domínguez, José

AU - A. Marchat, Laurence

AU - López-Camarillo, Cesar

AU - Mendoza-Hernández, Guillermo

AU - Sánchez-Espíndola, Esther

AU - Calzada, Fernando

AU - Ortega-Hernández, Alfredo

AU - Sánchez-Monroy, Virginia

AU - Ramírez-Moreno, Esther

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. Although several drugs are effective against E. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. Medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. Incomptine A isolated from Decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in vitro activity against E. histolytica trophozoites. However the molecular mechanisms involved in its antiprotozoal activity are still unknown. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that 21 E. histolytica proteins were differentially expressed in response to incomptine A treatment. Notably, three glycolytic enzymes, namely enolase, pyruvate:ferredoxin oxidoreductase and fructose-1,6-biphosphate aldolase, were down-regulated. Moreover, ultrastructural analysis of trophozoites through electronic microscopy showed an increased number of glycogen granules. Taken together, our data suggested that incomptine A could affect E. histolytica growth through alteration of its energy metabolism. © 2013 Elsevier Inc.

AB - Entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. Although several drugs are effective against E. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. Medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. Incomptine A isolated from Decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in vitro activity against E. histolytica trophozoites. However the molecular mechanisms involved in its antiprotozoal activity are still unknown. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that 21 E. histolytica proteins were differentially expressed in response to incomptine A treatment. Notably, three glycolytic enzymes, namely enolase, pyruvate:ferredoxin oxidoreductase and fructose-1,6-biphosphate aldolase, were down-regulated. Moreover, ultrastructural analysis of trophozoites through electronic microscopy showed an increased number of glycogen granules. Taken together, our data suggested that incomptine A could affect E. histolytica growth through alteration of its energy metabolism. © 2013 Elsevier Inc.

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Velázquez-Domínguez J, A. Marchat L, López-Camarillo C, Mendoza-Hernández G, Sánchez-Espíndola E, Calzada F et al. Effect of the sesquiterpene lactone incomptine A in the energy metabolism of Entamoeba histolytica. Experimental Parasitology. 2013 Nov 1;503-510. https://doi.org/10.1016/j.exppara.2013.08.015