TY - JOUR
T1 - Efecto del Medio Condicionado de la Línea Celular H9C2 Tratada con Dehidroepiandrosterona y Expuesta a Daño sobre la Motilidad de Células Troncales Mesenquimales
AU - Castro-Santos, G. X.
AU - Salas-Medina, J. L.
AU - López-Marure, R.
AU - Cáceres-Cortés, J. R.
AU - Parra-Barrera, A.
AU - Mera-Jiménez, E.
AU - Gutiérrez-Iglesias, G.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Cardiovascular diseases (CVD) are the leading cause of death worldwide. Mesenchymal Stem Cell (MSC) therapy is an alternative for patients who cannot recover with current treatments. Ensure movilization of MSC to the affected organs would represent an advantage for therapeutic management of CVD. Dehydroepiandrosterone (DHEA) is a hormone precursor whose levels decrease throughout life, which has been associated with the onset of CVD. Several studies have shown that DHEA consumption, prevents and improves heart condition, although it is not known if this is because an effect on cardiomyocytes is exercised on these cells and this, in turn, to CTM. The aim of this study was to determine the effect of conditioned medium from H9C2 cell line pretreated with DHEA and subjected to damage, on the motility of CTM, performing a wound healing assay. Pretreatment with DHEA and damage to H9C2 cell line, promotes motility of CTM. Stimulation of CTM motility by an indirect effect of DHEA could be a therapeutic strategy for heart damage.
AB - Cardiovascular diseases (CVD) are the leading cause of death worldwide. Mesenchymal Stem Cell (MSC) therapy is an alternative for patients who cannot recover with current treatments. Ensure movilization of MSC to the affected organs would represent an advantage for therapeutic management of CVD. Dehydroepiandrosterone (DHEA) is a hormone precursor whose levels decrease throughout life, which has been associated with the onset of CVD. Several studies have shown that DHEA consumption, prevents and improves heart condition, although it is not known if this is because an effect on cardiomyocytes is exercised on these cells and this, in turn, to CTM. The aim of this study was to determine the effect of conditioned medium from H9C2 cell line pretreated with DHEA and subjected to damage, on the motility of CTM, performing a wound healing assay. Pretreatment with DHEA and damage to H9C2 cell line, promotes motility of CTM. Stimulation of CTM motility by an indirect effect of DHEA could be a therapeutic strategy for heart damage.
KW - Cardiac damage
KW - Dehydroepiandrosterone
KW - Mesenchymal stem cells
KW - Motility
KW - Wound healing assay
UR - http://www.scopus.com/inward/record.url?scp=85019153691&partnerID=8YFLogxK
U2 - 10.17488/RMIB.38.1.21
DO - 10.17488/RMIB.38.1.21
M3 - Artículo
SN - 0188-9532
VL - 38
SP - 265
EP - 272
JO - Revista Mexicana de Ingenieria Biomedica
JF - Revista Mexicana de Ingenieria Biomedica
IS - 1
ER -