Effect of stirring speed on the production of phenolic secondary metabolites and growth of Buddleja cordata cells cultured in mechanically agitated bioreactor

Alicia Monserrat Vazquez-Marquez, Carmen Zepeda-Gómez, Cristina Burrola-Aguilar, Antonio Bernabé-Antonio, Aurelio Nieto-Trujillo, Francisco Cruz-Sosa, Mario Rodríguez-Monroy, María Elena Estrada-Zúñiga

Research output: Contribution to journalArticle

Abstract

Shake-flask in vitro culture of Buddleja cordata cells produces large amounts of biomass and synthetizes verbascoside (VB), linarin and hydroxycinnamic acids, bioactive phenolic secondary metabolites (PSMs). In this work, we determined the effect of stirring speed on the growth of and production of PSMs [total phenolic, phenylethanoid glycoside and flavonoid contents (PeC, PeGC and FC, respectively)] by B. cordata cells cultured in two bioreactors. Two different stirring speeds (120 and 400 rpm) were tested in two stirred-tank bioreactors: a 2 L bioreactor equipped with a ring diffuser (B2RD) and a 3 L bioreactor with a sintered diffuser (B3SD). Growth kinetics of B. cordata cells were measured in the bioreactors and shake-flask systems. The stirring speed and type of bioreactor affected phases, parameters of growth and production of PSMs. The highest production of biomass (13.62 g L−1) and PSMs [PeC of 64.63 mg gallic acid equivalents g−1 (mg GAE g−1); PeGC of 119.24 mg VB equivalents g−1 (mg VBE g−1); and FC of 5.02 mg quercetin equivalents g−1 (mg QE g−1)] occurred in B2RD at 400 rpm. These values were similar to the found in shake-flasks system. This work establishes the basis for bioprocess advances of B. cordata focused on the development of a sustainable strategy for the management of natural resources and as a source of bioactive PSMs on a large scale.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalPlant Cell, Tissue and Organ Culture
Volume139
Issue number1
DOIs
StatePublished - 1 Oct 2019

Fingerprint

Buddleja cordata
bioreactors
cultured cells
secondary metabolites
diffusers
verbascoside
coumaric acids
natural resource management
gallic acid
sustainable development
in vitro culture
quercetin
glycosides
biomass production
flavonoids
cells
kinetics
biomass

Keywords

  • Bioreactor
  • Buddleja cordata
  • Diffuser
  • Phenolic secondary metabolites
  • Stirring speed
  • Verbascoside

Cite this

Vazquez-Marquez, A. M., Zepeda-Gómez, C., Burrola-Aguilar, C., Bernabé-Antonio, A., Nieto-Trujillo, A., Cruz-Sosa, F., ... Estrada-Zúñiga, M. E. (2019). Effect of stirring speed on the production of phenolic secondary metabolites and growth of Buddleja cordata cells cultured in mechanically agitated bioreactor. Plant Cell, Tissue and Organ Culture, 139(1), 155-166. https://doi.org/10.1007/s11240-019-01673-9
Vazquez-Marquez, Alicia Monserrat ; Zepeda-Gómez, Carmen ; Burrola-Aguilar, Cristina ; Bernabé-Antonio, Antonio ; Nieto-Trujillo, Aurelio ; Cruz-Sosa, Francisco ; Rodríguez-Monroy, Mario ; Estrada-Zúñiga, María Elena. / Effect of stirring speed on the production of phenolic secondary metabolites and growth of Buddleja cordata cells cultured in mechanically agitated bioreactor. In: Plant Cell, Tissue and Organ Culture. 2019 ; Vol. 139, No. 1. pp. 155-166.
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Vazquez-Marquez, AM, Zepeda-Gómez, C, Burrola-Aguilar, C, Bernabé-Antonio, A, Nieto-Trujillo, A, Cruz-Sosa, F, Rodríguez-Monroy, M & Estrada-Zúñiga, ME 2019, 'Effect of stirring speed on the production of phenolic secondary metabolites and growth of Buddleja cordata cells cultured in mechanically agitated bioreactor', Plant Cell, Tissue and Organ Culture, vol. 139, no. 1, pp. 155-166. https://doi.org/10.1007/s11240-019-01673-9

Effect of stirring speed on the production of phenolic secondary metabolites and growth of Buddleja cordata cells cultured in mechanically agitated bioreactor. / Vazquez-Marquez, Alicia Monserrat; Zepeda-Gómez, Carmen; Burrola-Aguilar, Cristina; Bernabé-Antonio, Antonio; Nieto-Trujillo, Aurelio; Cruz-Sosa, Francisco; Rodríguez-Monroy, Mario; Estrada-Zúñiga, María Elena.

In: Plant Cell, Tissue and Organ Culture, Vol. 139, No. 1, 01.10.2019, p. 155-166.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of stirring speed on the production of phenolic secondary metabolites and growth of Buddleja cordata cells cultured in mechanically agitated bioreactor

AU - Vazquez-Marquez, Alicia Monserrat

AU - Zepeda-Gómez, Carmen

AU - Burrola-Aguilar, Cristina

AU - Bernabé-Antonio, Antonio

AU - Nieto-Trujillo, Aurelio

AU - Cruz-Sosa, Francisco

AU - Rodríguez-Monroy, Mario

AU - Estrada-Zúñiga, María Elena

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Shake-flask in vitro culture of Buddleja cordata cells produces large amounts of biomass and synthetizes verbascoside (VB), linarin and hydroxycinnamic acids, bioactive phenolic secondary metabolites (PSMs). In this work, we determined the effect of stirring speed on the growth of and production of PSMs [total phenolic, phenylethanoid glycoside and flavonoid contents (PeC, PeGC and FC, respectively)] by B. cordata cells cultured in two bioreactors. Two different stirring speeds (120 and 400 rpm) were tested in two stirred-tank bioreactors: a 2 L bioreactor equipped with a ring diffuser (B2RD) and a 3 L bioreactor with a sintered diffuser (B3SD). Growth kinetics of B. cordata cells were measured in the bioreactors and shake-flask systems. The stirring speed and type of bioreactor affected phases, parameters of growth and production of PSMs. The highest production of biomass (13.62 g L−1) and PSMs [PeC of 64.63 mg gallic acid equivalents g−1 (mg GAE g−1); PeGC of 119.24 mg VB equivalents g−1 (mg VBE g−1); and FC of 5.02 mg quercetin equivalents g−1 (mg QE g−1)] occurred in B2RD at 400 rpm. These values were similar to the found in shake-flasks system. This work establishes the basis for bioprocess advances of B. cordata focused on the development of a sustainable strategy for the management of natural resources and as a source of bioactive PSMs on a large scale.

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KW - Buddleja cordata

KW - Diffuser

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