Effect of restraint stress on the population of intestinal intraepithelial lymphocytes in mice

Adriana Jarillo-Luna, Víctor Rivera-Aguilar, Beatriz Elina Martìnez-Carrillo, Elizabeth Barbosa-Cabrera, Humberto Reyna Garfias, Rafael Campos-Rodríguez

Research output: Contribution to journalArticle

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Abstract

The impact of restraint stress on the intestinal immune system, particularly on intestinal intraepithelial lymphocytes (i-IEL), has not been described in detail. Thus, the purpose of this study was to assess the effects of restraint stress, including those produced by increases in glucocorticoids and catecholamines, on the population of i-IEL. Mice were exposed to 1 or 4 h restraint stress for 4 day, and the number of IEL in the mucosa of the proximal small intestine was determined by immunohistochemistry. The effects of restraint were also analyzed in mice submitted to different procedures: adrenalectomy, chemical sympathectomy, and treatment with a glucocorticoid antagonist (RU486), dexamethasone, and epinephrine. The main findings were that: (1) chronic restraint-stress reduced the i-IEl population in the small intestine; (2) adrenalectomy, treatment with RU-486 and chemical sympathectomy decreased the number of γδ, CD4+ and CD8+ T cells in non-stressed groups; (3) dexamethasone reduced the number of γδ and CD8+ T cells, and (4) epinephrine reduced the number of γδ, CD4+ and CD8+ T cells. These results demonstrated that restraint stress decreased the number of i-IEL in the proximal small intestine of mice, mainly by the combined action of higher concentrations of catecholamines and glucocorticoids, and that lower concentrations of glucocorticoids and catecholamines in unstressed mice preserved the population of i-IEL. © 2007 Elsevier Inc. All rights reserved.
Original languageAmerican English
Pages (from-to)265-275
Number of pages237
JournalBrain, Behavior, and Immunity
DOIs
StatePublished - 1 Jan 2008

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Lymphocytes
Glucocorticoids
Chemical Sympathectomy
T-cells
Small Intestine
Catecholamines
Adrenalectomy
T-Lymphocytes
Dexamethasone
Population
Epinephrine
Mifepristone
Immune system
immune system
Immune System
Mucous Membrane
Immunohistochemistry
effect
Therapeutics

Cite this

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title = "Effect of restraint stress on the population of intestinal intraepithelial lymphocytes in mice",
abstract = "The impact of restraint stress on the intestinal immune system, particularly on intestinal intraepithelial lymphocytes (i-IEL), has not been described in detail. Thus, the purpose of this study was to assess the effects of restraint stress, including those produced by increases in glucocorticoids and catecholamines, on the population of i-IEL. Mice were exposed to 1 or 4 h restraint stress for 4 day, and the number of IEL in the mucosa of the proximal small intestine was determined by immunohistochemistry. The effects of restraint were also analyzed in mice submitted to different procedures: adrenalectomy, chemical sympathectomy, and treatment with a glucocorticoid antagonist (RU486), dexamethasone, and epinephrine. The main findings were that: (1) chronic restraint-stress reduced the i-IEl population in the small intestine; (2) adrenalectomy, treatment with RU-486 and chemical sympathectomy decreased the number of γδ, CD4+ and CD8+ T cells in non-stressed groups; (3) dexamethasone reduced the number of γδ and CD8+ T cells, and (4) epinephrine reduced the number of γδ, CD4+ and CD8+ T cells. These results demonstrated that restraint stress decreased the number of i-IEL in the proximal small intestine of mice, mainly by the combined action of higher concentrations of catecholamines and glucocorticoids, and that lower concentrations of glucocorticoids and catecholamines in unstressed mice preserved the population of i-IEL. {\circledC} 2007 Elsevier Inc. All rights reserved.",
author = "Adriana Jarillo-Luna and V{\'i}ctor Rivera-Aguilar and Mart{\`i}nez-Carrillo, {Beatriz Elina} and Elizabeth Barbosa-Cabrera and Garfias, {Humberto Reyna} and Rafael Campos-Rodr{\'i}guez",
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Effect of restraint stress on the population of intestinal intraepithelial lymphocytes in mice. / Jarillo-Luna, Adriana; Rivera-Aguilar, Víctor; Martìnez-Carrillo, Beatriz Elina; Barbosa-Cabrera, Elizabeth; Garfias, Humberto Reyna; Campos-Rodríguez, Rafael.

In: Brain, Behavior, and Immunity, 01.01.2008, p. 265-275.

Research output: Contribution to journalArticle

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T1 - Effect of restraint stress on the population of intestinal intraepithelial lymphocytes in mice

AU - Jarillo-Luna, Adriana

AU - Rivera-Aguilar, Víctor

AU - Martìnez-Carrillo, Beatriz Elina

AU - Barbosa-Cabrera, Elizabeth

AU - Garfias, Humberto Reyna

AU - Campos-Rodríguez, Rafael

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