TY - JOUR
T1 - Effect of pregnancy and diabetes on vascular receptors for angiotensin II.
AU - Velazquez-Roman, Jorge A.
AU - Villafaña, Santiago
AU - Lopez Sanchez, Pedro
AU - Fernandez-Vallín, Eulalia
AU - Bobadilla Lugo, Rosa A.
N1 - Funding Information:
This research was partially supported by a Comision de Operacion y Fomento de Actividades Academicas (COFAA) grant.
PY - 2011
Y1 - 2011
N2 - Both gestation and diabetes mellitus (DM) are conditions associated with an increased participation of renin-angiotensin system (RAS) as well as with changes in the vascular response to angiotensin II (Ang II). We sought to establish the impact of gestational diabetes mellitus (GDM) on Ang II- induced vasoconstriction and its correlation with the expression of angiotensin II receptors (AT(1)R, AT(2) R). Female Wistar rats, virgin, or on day 3 after mating, received randomly streptozotocin (STZ) 60 mg/kgor saline ip.Streptozotocin-treated animals developed hyperglycemia (25.6 ± 1.42 mM). Ang II-induced vasoconstriction was evaluated on isolated aortas with (e+) and without (e-) endothelium and the protein expression of AT(1)R and AT(2)R was measured by western blot. Gestational diabetes mellitus significantly increased vasoconstriction with respect to all the other groups. Changes were observed only on e+ vessels. Interestingly, GDM moved AT(1)R: AT(2)R balance towards AT(1) R, while both pregnancy and diabetes towards AT(2)R expression. In conclusion, GDM increases the possibility of an hypertensive complication by an increased AT(1)R expression.
AB - Both gestation and diabetes mellitus (DM) are conditions associated with an increased participation of renin-angiotensin system (RAS) as well as with changes in the vascular response to angiotensin II (Ang II). We sought to establish the impact of gestational diabetes mellitus (GDM) on Ang II- induced vasoconstriction and its correlation with the expression of angiotensin II receptors (AT(1)R, AT(2) R). Female Wistar rats, virgin, or on day 3 after mating, received randomly streptozotocin (STZ) 60 mg/kgor saline ip.Streptozotocin-treated animals developed hyperglycemia (25.6 ± 1.42 mM). Ang II-induced vasoconstriction was evaluated on isolated aortas with (e+) and without (e-) endothelium and the protein expression of AT(1)R and AT(2)R was measured by western blot. Gestational diabetes mellitus significantly increased vasoconstriction with respect to all the other groups. Changes were observed only on e+ vessels. Interestingly, GDM moved AT(1)R: AT(2)R balance towards AT(1) R, while both pregnancy and diabetes towards AT(2)R expression. In conclusion, GDM increases the possibility of an hypertensive complication by an increased AT(1)R expression.
UR - http://www.scopus.com/inward/record.url?scp=80053330255&partnerID=8YFLogxK
U2 - 10.3109/10641963.2010.531843
DO - 10.3109/10641963.2010.531843
M3 - Artículo
C2 - 21473733
SN - 1064-1963
VL - 33
SP - 167
EP - 173
JO - Clinical and Experimental Hypertension
JF - Clinical and Experimental Hypertension
IS - 3
ER -