Effect of immersion cycles on growth, phenolics content, and antioxidant properties of Castilleja tenuiflora shoots

Raúl Valdez-Tapia, Jacqueline Capataz-Tafur, Alma Rosa López-Laredo, José Luis Trejo-Espino, Gabriela Trejo-Tapia

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

© 2014, The Society for In Vitro Biology. Castilleja tenuiflora, a species highly valued for its medicinal properties, is threatened in the wild. We evaluated the effects of six different immersion cycles in a temporary immersion bioreactor on C. tenuiflora shoot growth, proliferation rate, phenolics content, flavonoid content, and antioxidant activity. We also evaluated the regeneration capacity of the shoots. The highest proliferation rate (nine shoots per explant) was obtained using an immersion cycle of 5 min every 12 h, and the longest shoots (38.8 ± 1.9 mm) were obtained using an immersion cycle of 5 min every 24 h. Shoots obtained from immersion cycles of 30 min every 24 h or 5 min every 24 h showed 100% rooting efficiency. Shoots obtained from immersion cycles of 30 min every 3 h or 30 min every 12 h accumulated H2O2, developed abnormal stomata, and showed symptoms of hyperhydricity. These characteristics were associated with a low survival rate (16–80%) when the plants were transferred to potting mix. The shoots from an immersion cycle of 30 min every 24 h showed the highest total phenolics content, which coincided with the highest antioxidant activity in the 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) free-radical scavenging assay (161.74 ± 10.06 μmol Trolox/g dry weight (DW)). The shoots from an immersion cycle of 5 min every 24 h showed the highest activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay, and those from an immersion cycle of 5 min every 3 h showed the strongest reducing power. These results show that temporary immersion culture represents a reliable and efficient method for in vitro micropropagation of C. tenuiflora.
Original languageAmerican English
Pages (from-to)471-477
Number of pages423
JournalIn Vitro Cellular and Developmental Biology - Plant
DOIs
StatePublished - 1 Aug 2014

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Orobanchaceae
Castilleja
Scavenging
Immersion
Antioxidants
Free radicals
Free Radicals
Assays
antioxidants
Flavonoids
shoots
Sulfonic Acids
Growth
Bioreactors
Salts
Acids
antioxidant activity
hyperhydricity
medicinal properties
sulfonic acid

Cite this

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title = "Effect of immersion cycles on growth, phenolics content, and antioxidant properties of Castilleja tenuiflora shoots",
abstract = "{\circledC} 2014, The Society for In Vitro Biology. Castilleja tenuiflora, a species highly valued for its medicinal properties, is threatened in the wild. We evaluated the effects of six different immersion cycles in a temporary immersion bioreactor on C. tenuiflora shoot growth, proliferation rate, phenolics content, flavonoid content, and antioxidant activity. We also evaluated the regeneration capacity of the shoots. The highest proliferation rate (nine shoots per explant) was obtained using an immersion cycle of 5 min every 12 h, and the longest shoots (38.8 ± 1.9 mm) were obtained using an immersion cycle of 5 min every 24 h. Shoots obtained from immersion cycles of 30 min every 24 h or 5 min every 24 h showed 100{\%} rooting efficiency. Shoots obtained from immersion cycles of 30 min every 3 h or 30 min every 12 h accumulated H2O2, developed abnormal stomata, and showed symptoms of hyperhydricity. These characteristics were associated with a low survival rate (16–80{\%}) when the plants were transferred to potting mix. The shoots from an immersion cycle of 30 min every 24 h showed the highest total phenolics content, which coincided with the highest antioxidant activity in the 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) free-radical scavenging assay (161.74 ± 10.06 μmol Trolox/g dry weight (DW)). The shoots from an immersion cycle of 5 min every 24 h showed the highest activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay, and those from an immersion cycle of 5 min every 3 h showed the strongest reducing power. These results show that temporary immersion culture represents a reliable and efficient method for in vitro micropropagation of C. tenuiflora.",
author = "Ra{\'u}l Valdez-Tapia and Jacqueline Capataz-Tafur and L{\'o}pez-Laredo, {Alma Rosa} and Trejo-Espino, {Jos{\'e} Luis} and Gabriela Trejo-Tapia",
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Effect of immersion cycles on growth, phenolics content, and antioxidant properties of Castilleja tenuiflora shoots. / Valdez-Tapia, Raúl; Capataz-Tafur, Jacqueline; López-Laredo, Alma Rosa; Trejo-Espino, José Luis; Trejo-Tapia, Gabriela.

In: In Vitro Cellular and Developmental Biology - Plant, 01.08.2014, p. 471-477.

Research output: Contribution to journalArticle

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