© 2018 Florida Entomological Society. All rights reserved. The biological activity of 4 Mexican isolates (SeSIN6, SeSIN8, SeSLP6, and SeSLP8) of the homologous multiple nucleopolyhedrovirus SeMNPV and their effects on the development of the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), were studied. An exotic isolate of SeMNPV from the United States (SeUS2) was used as a reference. Early third-instar larvae were inoculated with 5 × 106 occlusion bodies per mL, resulting in 78% mortality for SeUS2 and approximately 90% mortality for each of the 4 Mexican isolates at 144 h post-inoculation. At 168 h postinoculation, 100% mortality was obtained in all cases. All of the isolates, including SeUS2, significantly reduced the body weights of S. exigua larvae compared with the control larvae; however, at 120 h post-inoculation, the differences among isolates were not significant. All of the isolates reduced the development time of third-instar S. exigua larvae (range: 1.2- to 1.6-fold) compared with the control larvae. An independent experiment was performed to determine production of occlusion bodies and mean time to death. Third-instar S. exigua larvae (15 h after molting) were inoculated with each isolate using the same concentration mentioned above. The occlusion body production rate was similar among all isolates. The isolates SeUS2, SeSLP6, and SeSLP8 yielded the fastest mortality (range: 187-191 h). Thus, the biological activities of the Mexican SeMNPV isolates were similar to the activity of the exotic isolate, indicating that these indigenous viruses are promising for the biological control of S. exigua in Mexico.
Luna-Espino, J. C., Castrejón-Gómez, V. R., Pineda, S., Figueroa, J. I., & Martínez, A. M. (2018). Effect of Four Multiple Nucleopolyhedrovirus Isolates on the Larval Mortality and Development of Spodoptera exigua (Lepidoptera: Noctuidae): Determination of Virus Production and Mean Time to Death. Florida Entomologist, 153-159. https://doi.org/10.1653/024.101.0201