TY - JOUR
T1 - Effect of chitosan on growth and plasma membrane properties of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill
AU - García-Rincón, J.
AU - Vega-Pérez, J.
AU - Guerra-Sánchez, M. G.
AU - Hernández-Lauzardo, A. N.
AU - Peña-Díaz, A.
AU - Velázquez-Del Valle, M. G.
N1 - Funding Information:
The authors are grateful to the National Polytechnic Institute for financial support. We also thanks for the technical assistance in the K+ efflux experiments to Ph.D Martha Calahorra Fuentes and QFB Norma Silvia Sánchez from the Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
PY - 2010/7
Y1 - 2010/7
N2 - The effect of chitosan (0.5, 1.0 and 2.0 mg ml-1) on mycelium growth, potassium efflux, pH of the incubation medium, and activity of plasma membrane H+-ATPase of Rhizopus stolonifer was evaluated. The mycelium growth of R. stolonifer was reduced on media amended with chitosan at 1.0 and 2.0 mg ml-1. The highest antifungal index (65%) was obtained on media containing chitosan at 2.0mgml-1. Potassium efflux induced by the addition of the three chitosan concentrations was demonstrated. The highest potassium effluxes were observed with addition of chitosan at 2.0 mg ml-1. R. stolonifer grown 72 h on Potato Dextrose Broth without chitosan generated a decrease in the medium's pH from 5.4 to 4.8. However, the addition of chitosan at all concentrations caused increases on pH of medium cultures. The highest pH increases were observed on media amended chitosan at 2.0 mg ml-1 resulting in the raising of pH level to 6.9. Additionally, there was an important inhibitory effect of chitosan (1.0 and 2.0 mg ml-1) on H+-ATPase activity in the plasma membrane of R. stolonifer (32.70%).
AB - The effect of chitosan (0.5, 1.0 and 2.0 mg ml-1) on mycelium growth, potassium efflux, pH of the incubation medium, and activity of plasma membrane H+-ATPase of Rhizopus stolonifer was evaluated. The mycelium growth of R. stolonifer was reduced on media amended with chitosan at 1.0 and 2.0 mg ml-1. The highest antifungal index (65%) was obtained on media containing chitosan at 2.0mgml-1. Potassium efflux induced by the addition of the three chitosan concentrations was demonstrated. The highest potassium effluxes were observed with addition of chitosan at 2.0 mg ml-1. R. stolonifer grown 72 h on Potato Dextrose Broth without chitosan generated a decrease in the medium's pH from 5.4 to 4.8. However, the addition of chitosan at all concentrations caused increases on pH of medium cultures. The highest pH increases were observed on media amended chitosan at 2.0 mg ml-1 resulting in the raising of pH level to 6.9. Additionally, there was an important inhibitory effect of chitosan (1.0 and 2.0 mg ml-1) on H+-ATPase activity in the plasma membrane of R. stolonifer (32.70%).
KW - Chitosan
KW - H-ATPase activity
KW - Medium pH
KW - Potassium efflux
KW - R. stolonifer
UR - http://www.scopus.com/inward/record.url?scp=77954035776&partnerID=8YFLogxK
U2 - 10.1016/j.pestbp.2010.03.008
DO - 10.1016/j.pestbp.2010.03.008
M3 - Artículo
SN - 0048-3575
VL - 97
SP - 275
EP - 278
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
IS - 3
ER -