TY - JOUR
T1 - Effect of agitation rate on biomass and protease production by a marine bacterium Vibrio harveyi cultured in a fermentor
AU - Estrada-Badillo, César
AU - Márquez-Rocha, Facundo J.
N1 - Funding Information:
Cesar Estrada Badillo is a fellow of CONACYT no. 113533. The project was supported by an Internal Grand of CICESE, no. 625105. We wish to thank Dr. Leonardo Lizarraga and Dra. Ilyina for the use of some equipment.
PY - 2003/3
Y1 - 2003/3
N2 - A marine bacterium Vibrio harveyi was adapted to grow and produce extracellular proteases in a seawater/Zobell-based medium, supplemented with skim milk under different hydrodynamic conditions, namely agitation and aeration rates. The addition of skim milk to Zobell medium enhanced fivefold the extracellular enzyme production. Protease production seemed to take place after maximum luminescence had been produced. Specific growth rate increased as a consequence of increasing agitation rates. The maximum activity of 4.28 units mg-1 protein were formed with 700 rev min-1 and 0.5 v/v/m. Protease activity detected has a molecular weight of 34 kDa. Another minor band of protease activity was found at 40 kDa.
AB - A marine bacterium Vibrio harveyi was adapted to grow and produce extracellular proteases in a seawater/Zobell-based medium, supplemented with skim milk under different hydrodynamic conditions, namely agitation and aeration rates. The addition of skim milk to Zobell medium enhanced fivefold the extracellular enzyme production. Protease production seemed to take place after maximum luminescence had been produced. Specific growth rate increased as a consequence of increasing agitation rates. The maximum activity of 4.28 units mg-1 protein were formed with 700 rev min-1 and 0.5 v/v/m. Protease activity detected has a molecular weight of 34 kDa. Another minor band of protease activity was found at 40 kDa.
KW - Extracellular proteases
KW - Hydrodynamic conditions
KW - Marine bacteria
KW - Marine fermentation
UR - http://www.scopus.com/inward/record.url?scp=0037731585&partnerID=8YFLogxK
U2 - 10.1023/A:1023257108488
DO - 10.1023/A:1023257108488
M3 - Artículo
AN - SCOPUS:0037731585
SN - 0959-3993
VL - 19
SP - 129
EP - 133
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 2
ER -