Effect of agitation rate on biomass and protease production by a marine bacterium Vibrio harveyi cultured in a fermentor

César Estrada-Badillo, Facundo J. Márquez-Rocha

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A marine bacterium Vibrio harveyi was adapted to grow and produce extracellular proteases in a seawater/Zobell-based medium, supplemented with skim milk under different hydrodynamic conditions, namely agitation and aeration rates. The addition of skim milk to Zobell medium enhanced fivefold the extracellular enzyme production. Protease production seemed to take place after maximum luminescence had been produced. Specific growth rate increased as a consequence of increasing agitation rates. The maximum activity of 4.28 units mg-1 protein were formed with 700 rev min-1 and 0.5 v/v/m. Protease activity detected has a molecular weight of 34 kDa. Another minor band of protease activity was found at 40 kDa.

Original languageEnglish
Pages (from-to)129-133
Number of pages5
JournalWorld Journal of Microbiology and Biotechnology
Volume19
Issue number2
DOIs
StatePublished - Mar 2003
Externally publishedYes

Keywords

  • Extracellular proteases
  • Hydrodynamic conditions
  • Marine bacteria
  • Marine fermentation

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