TY - JOUR
T1 - Early transcriptional responses to chilling stress in tomato fruit with hot water pre-treatment
AU - Cruz-Mendívil, Abraham
AU - López-Valenzuela, José A.
AU - Calderón-Vázquez, Carlos L.
AU - Vega-García, Misael O.
AU - Reyes-Moreno, Cuauhtémoc
AU - Valdez-Ortiz, Angel
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - We previously demonstrated the effectiveness of a hot water (HW) treatment (40°C for 7min) to reduce chilling injury (CI) in tomato fruit and described the global transcriptional changes associated with CI tolerance and susceptibility after 2 weeks of cold storage. In this study we describe the early transcriptional responses of HW treated and non-treated tomato fruit after a short-term cold storage (2 and 24h at 5°C). RNA-Seq analysis detected a large number of differentially expressed genes that varied from 575 (control fruit after 2h at 5°C) to 5100 (HW treated fruit after 24h at 5°C). The protective effect of HW treatment against chilling stress was related first with the up-regulation of AP2/EREBP and C2H2-type zinc finger transcription factors, which are known to induce the expression of cold-regulated genes, and second with the up-regulation of chaperonins and peptidyl-prolyl cis-trans isomerases, which prevent the denaturation and aggregation of proteins. Also, some genes related to pathogen resistance (TIR, NBS and LRR families) were up-regulated in HW treated fruit after chilling, suggesting a crosstalk between biotic and abiotic stress responses. Transcriptional changes that were induced in HW treated fruit at early stages of chilling and maintained after long-term cold storage included the up-regulation of genes related to heat stress and the down-regulation of genes related to cell wall degradation.
AB - We previously demonstrated the effectiveness of a hot water (HW) treatment (40°C for 7min) to reduce chilling injury (CI) in tomato fruit and described the global transcriptional changes associated with CI tolerance and susceptibility after 2 weeks of cold storage. In this study we describe the early transcriptional responses of HW treated and non-treated tomato fruit after a short-term cold storage (2 and 24h at 5°C). RNA-Seq analysis detected a large number of differentially expressed genes that varied from 575 (control fruit after 2h at 5°C) to 5100 (HW treated fruit after 24h at 5°C). The protective effect of HW treatment against chilling stress was related first with the up-regulation of AP2/EREBP and C2H2-type zinc finger transcription factors, which are known to induce the expression of cold-regulated genes, and second with the up-regulation of chaperonins and peptidyl-prolyl cis-trans isomerases, which prevent the denaturation and aggregation of proteins. Also, some genes related to pathogen resistance (TIR, NBS and LRR families) were up-regulated in HW treated fruit after chilling, suggesting a crosstalk between biotic and abiotic stress responses. Transcriptional changes that were induced in HW treated fruit at early stages of chilling and maintained after long-term cold storage included the up-regulation of genes related to heat stress and the down-regulation of genes related to cell wall degradation.
KW - AP2/EREBP
KW - Cell wall
KW - Chilling injury
KW - Heat shock
KW - RNA-Seq
KW - Solanum lycopersicum
UR - http://www.scopus.com/inward/record.url?scp=84938506844&partnerID=8YFLogxK
U2 - 10.1016/j.postharvbio.2015.06.015
DO - 10.1016/j.postharvbio.2015.06.015
M3 - Artículo
SN - 0925-5214
VL - 109
SP - 137
EP - 144
JO - Postharvest Biology and Technology
JF - Postharvest Biology and Technology
ER -