Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG

Carlos G. García-Tovar, José Luna, Raúl Mena, Carlos I. Soto-Zárate, Rafael Cortés, Armando Pérez, Gloria León-Avila, Dominique Mornet, Alvaro Rendón, José Manuel Hernández

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Dp71 is the most abundant product of the dmd gene in the brain. There are at least 2 isoforms derived from alternative splicing of exon 78 (Dp71d, which contains exon 78 and Dp71f, the spliced isoform) but the precise localization and function of each isoform is still unknown. In the present study, we demonstrate by RT-PCR that the Dp71f isoform is present in an astrocytoma cell line U-373 MG, and its subcellular localization was determined in the cytoplasm, particularly in perinuclear areas, with lower amounts towards the periphery but increasing in the leader borders of lamellipodia and focal complexes. Double labeling indirect immunofluorescence showed that Dp71f colocalized with actin-like β-dystroglycan and β-1 integrin. We also demonstrated by triple labeling that Dp71f was colocalized with actin and two members of integrin complexes, α-actinin and vinculin, in focal complexes. Ventral plasma membranes were enriched and in those containing focal complex proteins, we found colocalization of Dp71f, actin and vinculin. It is concluded that U-373 MG cells express Dp71f as part of lamellipodia and focal complex proteins, and possibly connected via distroglycan complexes to integrin complexes.
Original languageAmerican English
Pages (from-to)245-254
Number of pages219
JournalActa Histochemica
DOIs
StatePublished - 1 Jan 2002
Externally publishedYes

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Dystrophin
Pseudopodia
Astrocytoma
Labeling
marking
Protein Isoforms
Integrins
Vinculin
proteins
Proteins
Actins
splicing
cytoplasm
Cell membranes
cells
borders
cultured cells
genes
brain
Exons

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García-Tovar, C. G., Luna, J., Mena, R., Soto-Zárate, C. I., Cortés, R., Pérez, A., ... Hernández, J. M. (2002). Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG. Acta Histochemica, 245-254. https://doi.org/10.1078/0065-1281-00654
García-Tovar, Carlos G. ; Luna, José ; Mena, Raúl ; Soto-Zárate, Carlos I. ; Cortés, Rafael ; Pérez, Armando ; León-Avila, Gloria ; Mornet, Dominique ; Rendón, Alvaro ; Hernández, José Manuel. / Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG. In: Acta Histochemica. 2002 ; pp. 245-254.
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abstract = "Dp71 is the most abundant product of the dmd gene in the brain. There are at least 2 isoforms derived from alternative splicing of exon 78 (Dp71d, which contains exon 78 and Dp71f, the spliced isoform) but the precise localization and function of each isoform is still unknown. In the present study, we demonstrate by RT-PCR that the Dp71f isoform is present in an astrocytoma cell line U-373 MG, and its subcellular localization was determined in the cytoplasm, particularly in perinuclear areas, with lower amounts towards the periphery but increasing in the leader borders of lamellipodia and focal complexes. Double labeling indirect immunofluorescence showed that Dp71f colocalized with actin-like β-dystroglycan and β-1 integrin. We also demonstrated by triple labeling that Dp71f was colocalized with actin and two members of integrin complexes, α-actinin and vinculin, in focal complexes. Ventral plasma membranes were enriched and in those containing focal complex proteins, we found colocalization of Dp71f, actin and vinculin. It is concluded that U-373 MG cells express Dp71f as part of lamellipodia and focal complex proteins, and possibly connected via distroglycan complexes to integrin complexes.",
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García-Tovar, CG, Luna, J, Mena, R, Soto-Zárate, CI, Cortés, R, Pérez, A, León-Avila, G, Mornet, D, Rendón, A & Hernández, JM 2002, 'Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG', Acta Histochemica, pp. 245-254. https://doi.org/10.1078/0065-1281-00654

Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG. / García-Tovar, Carlos G.; Luna, José; Mena, Raúl; Soto-Zárate, Carlos I.; Cortés, Rafael; Pérez, Armando; León-Avila, Gloria; Mornet, Dominique; Rendón, Alvaro; Hernández, José Manuel.

In: Acta Histochemica, 01.01.2002, p. 245-254.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG

AU - García-Tovar, Carlos G.

AU - Luna, José

AU - Mena, Raúl

AU - Soto-Zárate, Carlos I.

AU - Cortés, Rafael

AU - Pérez, Armando

AU - León-Avila, Gloria

AU - Mornet, Dominique

AU - Rendón, Alvaro

AU - Hernández, José Manuel

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Dp71 is the most abundant product of the dmd gene in the brain. There are at least 2 isoforms derived from alternative splicing of exon 78 (Dp71d, which contains exon 78 and Dp71f, the spliced isoform) but the precise localization and function of each isoform is still unknown. In the present study, we demonstrate by RT-PCR that the Dp71f isoform is present in an astrocytoma cell line U-373 MG, and its subcellular localization was determined in the cytoplasm, particularly in perinuclear areas, with lower amounts towards the periphery but increasing in the leader borders of lamellipodia and focal complexes. Double labeling indirect immunofluorescence showed that Dp71f colocalized with actin-like β-dystroglycan and β-1 integrin. We also demonstrated by triple labeling that Dp71f was colocalized with actin and two members of integrin complexes, α-actinin and vinculin, in focal complexes. Ventral plasma membranes were enriched and in those containing focal complex proteins, we found colocalization of Dp71f, actin and vinculin. It is concluded that U-373 MG cells express Dp71f as part of lamellipodia and focal complex proteins, and possibly connected via distroglycan complexes to integrin complexes.

AB - Dp71 is the most abundant product of the dmd gene in the brain. There are at least 2 isoforms derived from alternative splicing of exon 78 (Dp71d, which contains exon 78 and Dp71f, the spliced isoform) but the precise localization and function of each isoform is still unknown. In the present study, we demonstrate by RT-PCR that the Dp71f isoform is present in an astrocytoma cell line U-373 MG, and its subcellular localization was determined in the cytoplasm, particularly in perinuclear areas, with lower amounts towards the periphery but increasing in the leader borders of lamellipodia and focal complexes. Double labeling indirect immunofluorescence showed that Dp71f colocalized with actin-like β-dystroglycan and β-1 integrin. We also demonstrated by triple labeling that Dp71f was colocalized with actin and two members of integrin complexes, α-actinin and vinculin, in focal complexes. Ventral plasma membranes were enriched and in those containing focal complex proteins, we found colocalization of Dp71f, actin and vinculin. It is concluded that U-373 MG cells express Dp71f as part of lamellipodia and focal complex proteins, and possibly connected via distroglycan complexes to integrin complexes.

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