TY - JOUR
T1 - DNA damage and oxidative stress induced by acetylsalicylic acid in Daphnia magna
AU - Gómez-Oliván, Leobardo Manuel
AU - Galar-Martínez, Marcela
AU - Islas-Flores, Hariz
AU - García-Medina, Sandra
AU - Sanjuan-Reyes, Nely
N1 - Funding Information:
This study was made possible by support from the Consejo Nacional de Ciencia y Tecnología (CONACyT-Mexico, Project 151665 ).
PY - 2014/8
Y1 - 2014/8
N2 - Acetylsalicylic acid is a nonsteroidal anti-inflammatory widely used due to its low cost and high effectiveness. This compound has been found in water bodies worldwide and is toxic to aquatic organisms; nevertheless its capacity to induce oxidative stress in bioindicators like Daphnia magna remains unknown. This study aimed to evaluate toxicity in D. magna induced by acetylsalicylic acid in water, using oxidative stress and DNA damage biomarkers. An acute toxicity test was conducted in order to determine the median lethal concentration (48-h LC50) and the concentrations to be used in the subsequent subacute toxicity test in which the following biomarkers were evaluated: lipid peroxidation, oxidized protein content, activity of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, and level of DNA damage. Lipid peroxidation level and oxidized protein content were significantly increased (p < 0.05), and antioxidant enzymes significantly altered with respect to controls; while the DNA damage were significantly increased (p < 0.05) too. In conclusion, acetylsalicylic acid induces oxidative stress and DNA damage in D. magna.
AB - Acetylsalicylic acid is a nonsteroidal anti-inflammatory widely used due to its low cost and high effectiveness. This compound has been found in water bodies worldwide and is toxic to aquatic organisms; nevertheless its capacity to induce oxidative stress in bioindicators like Daphnia magna remains unknown. This study aimed to evaluate toxicity in D. magna induced by acetylsalicylic acid in water, using oxidative stress and DNA damage biomarkers. An acute toxicity test was conducted in order to determine the median lethal concentration (48-h LC50) and the concentrations to be used in the subsequent subacute toxicity test in which the following biomarkers were evaluated: lipid peroxidation, oxidized protein content, activity of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, and level of DNA damage. Lipid peroxidation level and oxidized protein content were significantly increased (p < 0.05), and antioxidant enzymes significantly altered with respect to controls; while the DNA damage were significantly increased (p < 0.05) too. In conclusion, acetylsalicylic acid induces oxidative stress and DNA damage in D. magna.
KW - Acetylsalicylic acid
KW - DNA damage
KW - Daphnia magna
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=84899869914&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2014.04.004
DO - 10.1016/j.cbpc.2014.04.004
M3 - Artículo
SN - 1532-0456
VL - 164
SP - 21
EP - 26
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
ER -