TY - JOUR
T1 - DNA barcoding and morphological taxonomy
T2 - identification of lanternfish (Myctophidae) larvae in the Gulf of Mexico
AU - Batta-Lona, Paola Gabriela
AU - Galindo-Sánchez, Clara E.
AU - Arteaga, Maria Clara
AU - Robles-Flores, Javier
AU - Jiménez-Rosenberg, Sylvia Patricia Aldeheid
N1 - Publisher Copyright:
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2019/2/17
Y1 - 2019/2/17
N2 - Accurate identification of fish larval stages is complicated and time-consuming due to the lack of diagnostic morphological characters, especially during early developmental stages. The distribution of lanternfish (Myctophidae) has been described based on the morphological identification of adult stages. Larvae of only a few species of Myctophidae have been described, and the description is not always precise. In this study, larvae were collected and morphologically identified as Diaphus mollis, Hygophum hygomii, H. reinhardtii, H. taaningi, Myctophum obtusirostre and M. selenops. The DNA barcode region of the mitochondrial cytochrome oxidase I gene (COI) was determined for all larvae. The COI sequences matched reference barcodes available in GenBank for 14 of the identified larvae. The remaining COI sequences matched reference barcodes for different species of Myctophidae including Centrobranchus nigroocellatus, Diogenichthys atlanticus and Lepidophanes guentheri. This effort demonstrated the importance of integrated morphological and molecular analysis of species diversity and distribution of the Myctophidae in the Gulf of México.
AB - Accurate identification of fish larval stages is complicated and time-consuming due to the lack of diagnostic morphological characters, especially during early developmental stages. The distribution of lanternfish (Myctophidae) has been described based on the morphological identification of adult stages. Larvae of only a few species of Myctophidae have been described, and the description is not always precise. In this study, larvae were collected and morphologically identified as Diaphus mollis, Hygophum hygomii, H. reinhardtii, H. taaningi, Myctophum obtusirostre and M. selenops. The DNA barcode region of the mitochondrial cytochrome oxidase I gene (COI) was determined for all larvae. The COI sequences matched reference barcodes available in GenBank for 14 of the identified larvae. The remaining COI sequences matched reference barcodes for different species of Myctophidae including Centrobranchus nigroocellatus, Diogenichthys atlanticus and Lepidophanes guentheri. This effort demonstrated the importance of integrated morphological and molecular analysis of species diversity and distribution of the Myctophidae in the Gulf of México.
KW - Cytochrome oxidase I
KW - DNA barcoding
KW - Gulf of Mexico
KW - Myctophidae
KW - larval fishes
UR - http://www.scopus.com/inward/record.url?scp=85060331842&partnerID=8YFLogxK
U2 - 10.1080/24701394.2018.1538364
DO - 10.1080/24701394.2018.1538364
M3 - Artículo
C2 - 30656991
SN - 2470-1394
VL - 30
SP - 375
EP - 383
JO - Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
JF - Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
IS - 2
ER -