Distinct lignocellulolytic enzymes produced by Trichoderma harzianum in response to different pretreated substrates

Yu Zhang, Ruonan Wang, Liang Liu, Entao Wang, Jinshui Yang, Hongli Yuan

Research output: Contribution to journalArticlepeer-review

Abstract

In order to optimize the composition of enzyme cocktail for improving the hydrolytic efficiency of lignocellulose, different substrates were tested as inducers for producing lignocellulolytic enzymes by Trichoderma harzianum EM0925 in this study. As results, ultrafine grinding or steam explosion pretreated substrates can induce T. harzianum EM0925 to secret holo lignocellulolytic enzymes; acid treated substrate can induce cellobiohydrolase; while alkali or sodium chlorite treated substrates can induce β-xylosidase specifically. Furthermore, the combination of enzyme cocktails with different hydrolysis characteristics can further improve the hydrolysis efficiency, since 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding treated corn stover at low enzyme dosage (1.2 mg proteins/g substrate). This study for the first time demonstrated an effective solution that specific-pretreated substrates can be used as inducers for specific enzyme production by T. harzianum, which provided new idea and potential strategy for the construction of highly-efficient lignocellulolytic enzyme cocktails.

Original languageEnglish
Article number128990
JournalBioresource Technology
Volume378
DOIs
StatePublished - Jun 2023
Externally publishedYes

Keywords

  • Corn stover
  • Induced enzyme
  • Lignocellulolytic enzyme cocktail
  • Pretreatment
  • Trichoderma harzianum

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