TY - JOUR
T1 - Distinct lignocellulolytic enzymes produced by Trichoderma harzianum in response to different pretreated substrates
AU - Zhang, Yu
AU - Wang, Ruonan
AU - Liu, Liang
AU - Wang, Entao
AU - Yang, Jinshui
AU - Yuan, Hongli
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/6
Y1 - 2023/6
N2 - In order to optimize the composition of enzyme cocktail for improving the hydrolytic efficiency of lignocellulose, different substrates were tested as inducers for producing lignocellulolytic enzymes by Trichoderma harzianum EM0925 in this study. As results, ultrafine grinding or steam explosion pretreated substrates can induce T. harzianum EM0925 to secret holo lignocellulolytic enzymes; acid treated substrate can induce cellobiohydrolase; while alkali or sodium chlorite treated substrates can induce β-xylosidase specifically. Furthermore, the combination of enzyme cocktails with different hydrolysis characteristics can further improve the hydrolysis efficiency, since 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding treated corn stover at low enzyme dosage (1.2 mg proteins/g substrate). This study for the first time demonstrated an effective solution that specific-pretreated substrates can be used as inducers for specific enzyme production by T. harzianum, which provided new idea and potential strategy for the construction of highly-efficient lignocellulolytic enzyme cocktails.
AB - In order to optimize the composition of enzyme cocktail for improving the hydrolytic efficiency of lignocellulose, different substrates were tested as inducers for producing lignocellulolytic enzymes by Trichoderma harzianum EM0925 in this study. As results, ultrafine grinding or steam explosion pretreated substrates can induce T. harzianum EM0925 to secret holo lignocellulolytic enzymes; acid treated substrate can induce cellobiohydrolase; while alkali or sodium chlorite treated substrates can induce β-xylosidase specifically. Furthermore, the combination of enzyme cocktails with different hydrolysis characteristics can further improve the hydrolysis efficiency, since 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding treated corn stover at low enzyme dosage (1.2 mg proteins/g substrate). This study for the first time demonstrated an effective solution that specific-pretreated substrates can be used as inducers for specific enzyme production by T. harzianum, which provided new idea and potential strategy for the construction of highly-efficient lignocellulolytic enzyme cocktails.
KW - Corn stover
KW - Induced enzyme
KW - Lignocellulolytic enzyme cocktail
KW - Pretreatment
KW - Trichoderma harzianum
UR - http://www.scopus.com/inward/record.url?scp=85151496807&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2023.128990
DO - 10.1016/j.biortech.2023.128990
M3 - Artículo
C2 - 37003454
AN - SCOPUS:85151496807
SN - 0960-8524
VL - 378
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 128990
ER -