TY - JOUR
T1 - Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm
AU - Garrido-Bazan, V.
AU - Mahuku, G.
AU - Bibbins-Martinez, M.
AU - Arroyo-Bacerra, A.
AU - Ángel Villalobos-López, M.
N1 - Publisher Copyright:
© 2018 Wageningen Academic Publishers.
PY - 2018
Y1 - 2018
N2 - Aspergillus flavus induced ear rots and subsequent contamination of maize (Zea mays L.) by aflatoxin is a serious food safety issue, especially in developing countries where the crop is mostly cultivated by smallholder famers for own consumption and income generation. A better understanding of the mechanisms of resistance could help breeders to develop resistant maize varieties. In this study, a set of six tropical maize inbred lines previously identified as resistant or susceptible under natural field conditions were evaluated for response to A. flavus colonisation and aflatoxin contamination. Fungal biomass was significantly higher (P<0.05) in susceptible than resistant maize inbred lines, and this was highly correlated (P=0.001) to aflatoxin levels. Maize inbred lines MRI, MR2 and MR3 had low fungal biomass and low aflatoxin levels, suggesting that resistance in these lines was mediated through restricted fungal colonisation and establishment. Among the three putatively resistant inbred lines mentioned above, MR2 had a relatively high colonisation compared to the other two lines, revealing that A. flavus could establish and colonise kernels that were injured during inoculation, but did not contain high levels of aflatoxin. This could signify the presence of host genes that interfere with the aflatoxin biosynthetic pathway.
AB - Aspergillus flavus induced ear rots and subsequent contamination of maize (Zea mays L.) by aflatoxin is a serious food safety issue, especially in developing countries where the crop is mostly cultivated by smallholder famers for own consumption and income generation. A better understanding of the mechanisms of resistance could help breeders to develop resistant maize varieties. In this study, a set of six tropical maize inbred lines previously identified as resistant or susceptible under natural field conditions were evaluated for response to A. flavus colonisation and aflatoxin contamination. Fungal biomass was significantly higher (P<0.05) in susceptible than resistant maize inbred lines, and this was highly correlated (P=0.001) to aflatoxin levels. Maize inbred lines MRI, MR2 and MR3 had low fungal biomass and low aflatoxin levels, suggesting that resistance in these lines was mediated through restricted fungal colonisation and establishment. Among the three putatively resistant inbred lines mentioned above, MR2 had a relatively high colonisation compared to the other two lines, revealing that A. flavus could establish and colonise kernels that were injured during inoculation, but did not contain high levels of aflatoxin. This could signify the presence of host genes that interfere with the aflatoxin biosynthetic pathway.
KW - Aflatoxins
KW - Aspergillus flavus
KW - Maize
KW - Resistance
UR - http://www.scopus.com/inward/record.url?scp=85046894421&partnerID=8YFLogxK
U2 - 10.3920/WMJ2017.2219
DO - 10.3920/WMJ2017.2219
M3 - Artículo
SN - 1875-0710
VL - 11
SP - 215
EP - 224
JO - World Mycotoxin Journal
JF - World Mycotoxin Journal
IS - 2
ER -