TY - JOUR
T1 - Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice
AU - Romeu, Eduardo J.Arista
AU - Escobedo, Galileo
AU - Campos-Espinosa, Adriana
AU - Romero-Bello, Ivette Irais
AU - Moreno-González, Javier
AU - Fabila-Bustos, Diego A.
AU - Reed, Alma Valor
AU - Isakina, Suren Stolik
AU - De La Rosa Vázquez, José Manuel
AU - Guzmán, Carolina
N1 - Publisher Copyright:
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Nonalcoholic fatty liver disease (NAFLD) ranges from steatosis to nonalcoholic steatohepatitis and cirrhosis. Liver biopsy, considered the gold standard to diagnose NAFLD, shows significantly high rates of interobserver variability. Thus there is a need to develop tools that accurately categorize mild and advanced grades of steatosis in order to identify patients at higher risk of developing chronic liver disease. Diffuse reflectance spectroscopy (DRS) has proved to be useful in grading liver fibrosis and cirrhosis, without having been implemented for steatosis. We aim to categorize early and advanced stages of liver steatosis in a methionine-choline deficient (MCD) mouse model. C57bl/6 mice are fed either methionine-choline control or MCD diet during 2 or 8 weeks to induce mild and advanced steatosis. Liver samples are obtained and steatosis is evaluated by oil red O staining. Diffuse reflectance spectra are directly measured on ex vivo liver specimens, in a wavelength range of 400 to 800 nm. DRS is able to discriminate between early or advanced steatosis and healthy hepatic tissue with negligible error while showing high average sensitivity and specificity (0.94 and 0.95, respectively). Our results suggest that liver steatosis can be accurately evaluated by DRS, highlighting the importance of applied spectroscopic methods in assessing NAFLD.
AB - Nonalcoholic fatty liver disease (NAFLD) ranges from steatosis to nonalcoholic steatohepatitis and cirrhosis. Liver biopsy, considered the gold standard to diagnose NAFLD, shows significantly high rates of interobserver variability. Thus there is a need to develop tools that accurately categorize mild and advanced grades of steatosis in order to identify patients at higher risk of developing chronic liver disease. Diffuse reflectance spectroscopy (DRS) has proved to be useful in grading liver fibrosis and cirrhosis, without having been implemented for steatosis. We aim to categorize early and advanced stages of liver steatosis in a methionine-choline deficient (MCD) mouse model. C57bl/6 mice are fed either methionine-choline control or MCD diet during 2 or 8 weeks to induce mild and advanced steatosis. Liver samples are obtained and steatosis is evaluated by oil red O staining. Diffuse reflectance spectra are directly measured on ex vivo liver specimens, in a wavelength range of 400 to 800 nm. DRS is able to discriminate between early or advanced steatosis and healthy hepatic tissue with negligible error while showing high average sensitivity and specificity (0.94 and 0.95, respectively). Our results suggest that liver steatosis can be accurately evaluated by DRS, highlighting the importance of applied spectroscopic methods in assessing NAFLD.
KW - diffuse reflectance spectroscopy
KW - nonalcoholic fatty liver disease
KW - steatosis
UR - http://www.scopus.com/inward/record.url?scp=85057555158&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.23.11.115005
DO - 10.1117/1.JBO.23.11.115005
M3 - Artículo
C2 - 30499266
AN - SCOPUS:85057555158
SN - 1083-3668
VL - 23
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 11
M1 - 115005
ER -